Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model  

作　　者：Jianghui Liu Liwei Yan Wei Yang Yong Lan Qiyu Zhu ongjie Xu Canbin Zheng Rui Guo 

机构地区：[1]Department of Emergency,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou,510080,China [2]Key Laboratory of Biomaterials of Guangdong Higher Education Institutes,Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development,Department of Biomedical Engineering,Jinan University,Guangzhou,510632,China [3]Beogene Biotech(Guangzhou)CO.,LTD,Guangzhou,510663,China [4]Department of Microsurgery and Orthopedic Trauma,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou,510080,China

出　　处：《Bioactive Materials》2019年第1期151-159,共9页生物活性材料（英文）

基　　金：supported financially by the Natural Science Foundation of China(51303064 and 31271019);the Science and Technology Program of Guangzhou(201601010270,2017010160489,201704030083);the Pearl River S&T Nova Program of Guangzhou(201710010155,201806010072);the Science and Technology Project of Guangdong province(2015A010101313,2017A050506011,2017B090911012,2018A050506040,2018A050506019,2018A050506021).

摘　　要：Diabetic foot ulcers(DFU),which may lead to lower extremity amputation,is one of the severe and chronic complications of diabetic mellitus.This study aims to develop,and use dressings based on Silk fibroin(SF)as the scaffold material,gelatin microspheres(GMs)as the carrier for the neurotensin(NT),a neuropeptide that acts as an inflammatory modulator in wound healing and NT as accelerate wound healing drug to treat DFU.We evaluated the wound healing processes and neo-tissue formation in rat diabetic model by macroscopic observation,histological observation(H&E staining and Masson's trichrome staining)and immunofluorescence analysis at 3,7,14,21 and 28 post-operation days.Our results show that the NT/GMs/SF group performance the best not only in macroscopic healing and less scars in 28 post-operation days,but also in fibroblast accumulation in tissue granulation,collagen expression and deposition at the wound site.From release profiles,we can know the GMs are a good carrier for control release drugs.The SEM results shows that the NT/GMs/SF dressings have an average pore size are 40–80μm and a porosity of∼85%,this pore size is suit for wound healing regeneration.These results suggest that the NT/GMs/SF dressings may work as an effective support for control release NT to promote DFU wound healing.

关 键 词：Diabetic foot ulcers(DFU) Silk fibroin(SF) Gelatin microspheres(GMs) Neurotensin(NT) Rat diabetic model 

分 类 号：R58[医药卫生—内分泌]