电针对控制性超排卵小鼠子宫内膜同源盒基因A10表达时相的影响  

Effect of Electrocupuncture on the Expression Phase of Endometrial Homologous Box Gene A10 in Mice with Controlled Ovarian Hyperstimulation

作　　者：张磊[1] 刘杰[2] 张英[1] 王芳[1] 李长雷[1] 刘骥才[1] 刘小亚 付艾妮[1] 游俊[1] 朱书秀[1] ZHANG Lei;LIU Jie;ZHANG Ying;WANG Fang;LI Chang-lei;LIU Ji-cai;LIU Xiao-ya;FU Ai-ni;YOU Jun;ZHU Shu-xiu(School of medicine,Jianghan University,Wuhan 430065,China;Hubei Maternal and Child Health Hospital,Wuhan 430070,China)

机构地区：[1]江汉大学医学院,武汉430056 [2]湖北省妇幼保健院,武汉430070

出　　处：《上海针灸杂志》2021年第2期213-218,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金面上项目(81674060);江汉大学省内特色学科提升项目(03100041)。

摘　　要：目的观察电针对控制性超排卵(COH)小鼠子宫内膜同源盒基因A10(HOXA10)表达时相的影响,探讨电针提高体外受精-胚胎移植(IVF-ET)临床妊娠率的作用机制。方法共有150只雌性和80只雄性小鼠,取50只雌性鼠为自然周期组,剩余100只雌鼠制备COH模型并随机分为COH组(50只)和电针组(50只)。各组内再根据妊娠天数分为D2、D3、D4、D5和D6共5个小组,每小组10只。取D6小组小鼠子宫,观察妊娠率及平均着床位点数;取D2、D3、D4和D5小组子宫内膜,采用Western blot和RT-PCR技术检测HOXA10、整合素αvβ3和白血病抑制因子(LIF)蛋白及mRNA的表达。结果COH组妊娠率较自然周期组低,子宫内膜HOXA10、整合素αvβ3和LIF表达峰值前移,D3小组高于D2、D4、D5小组(P<0.05,P<0.01)。经电针治疗后,HOXA10、整合素αvβ3和LIF表达峰值恢复至受孕第4天,较COH组推迟,临床妊娠率有增高趋势。结论电针调控COH小鼠子宫内膜HOXA10的表达时相,恢复正常种植窗期,是电针提高IVF-ET临床妊娠率的作用机制之一。Objective To investigate the effect of electroacupuncture on the expression phase of endometrial homologous box gene A10(HOXA10)in mice with controlled ovarian hyperstimulation(COH)and explore the mechanism of action by which electroacupuncture increases the clinical pregnancy rate after in vitro fertilization-embryo transfer(IVF-ET).Method Of a total of 150 female and 80 male mice,50 female mice constituted a natural cycle group and the remaining 100 female mice were used to make the COH models and randomized to a COH group(50 mice)and an electroacupuncture group(50 mice).According to the days of pregnancy,each group was again divided into 5 subgroups,D2,D3,D4,D5 and D6,10 mice in each subgroup.In the D6 subgroup of mice,the uterus was taken to observe the average number of embryo implantation sites.In the D2,D3,D4 and D5 subgroups of mice,the endometrium was taken and the expressions of HOXA10,integrinαvβ3,and leukemia inhibitory factor(LIF)protein and mRNA were determined by Western blot and RT-PCR.Result The pregnancy rate was lower in the COH group than that in the natural cycle group.The expression peaks of endometrial HOXA10,integrinαvβ3 and LIF shifted forward in the COH group compared with the natural cycle group and were higher in the D3 subgroup than in the D2,D4 and D5 subgroups(P<0.05,P<0.01).After electroacupuncture treatment,the expression peaks of HOXA10,integrinαvβ3 and LIF returned to the fourth day of pregnancy and delayed compared with the COH group,and the clinical pregnancy rate had an increasing trend.Conclusions Electroacupuncture regulates the expression phase of endometrial HOXA10 and restores the normal implantation window period,which is one of the mechanisms of action of electroacupuncture for increasing the clinical pregnancy rate after IVF-ET.

关 键 词：电针 控制性超排卵 同源盒基因A10 子宫内膜 妊娠 小鼠 辅助生殖技术 

分 类 号：R2-03[医药卫生—中医学]