Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering  

作　　者：Ting Su Mengying Zhang Qiankun Zeng Wenhao Pan Yijing Huang Yuna Qian Wei Dong Xiaoliang Qi Jianliang Shen 

机构地区：[1]State Key Laboratory of Ophthalmology,Optometry and Vision Science,School of Ophthalmology and Optometry,School of Biomedical Engineering,Wenzhou Medical University,Wenzhou,325027,China [2]Engineering Research Center of Clinical Functional Materials and Diagnosis&Treatment Devices of Zhejiang Province,Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,325001,China [3]School of Chemistry&Materials Engineering,Fuyang Normal University,Fuyang,236037,China [4]School of Chemical Engineering,Nanjing University of Science&Technology,Nanjing,210094,China

出　　处：《Bioactive Materials》2021年第3期579-588,共10页生物活性材料（英文）

基　　金：supported by National Natural Science Foundation of China(31800833, 21977081);Zhejiang Provincial Natural Science Foundation of China(LZ19H180001);University of Chinese Academy of Sciences(WIBEZD2017001-03 and WIUCASYJ2020001-2);Wenzhou Medical University(KYYW201901 and KYYW201906);Wenzhou Science and Technology Plan Project(Y20180071);Start-up Scientific Research Foundation of Wenzhou Medical University(KYQD20190513).

摘　　要：Polysaccharide hydrogels are widely used in tissue engineering because of their superior biocompatibility and low immunogenicity.However,many of these hydrogels are unrealistic for practical applications as the cost of raw materials is high,and the fabrication steps are tedious.This study focuses on the facile fabrication and optimization of agarose-polydopamine hydrogel(APG)scaffolds for skin wound healing.The first study objective was to evaluate the effects of polydopamine(PDA)on the mechanical properties,water holding capacity and cell adhesiveness of APG.We observed that APG showed decreased rigidity and increased water content with the addition of PDA.Most importantly,decreased rigidity translated into significant increase in cell adhesiveness.Next,the slow biodegradability and high biocompatibility of APG with the highest PDA content(APG3)was confirmed.In addition,APG3 promoted full-thickness skin defect healing by accelerating collagen deposition and promoting angiogenesis.Altogether,we have developed a straightforward and efficient strategy to construct functional APG scaffold for skin tissue engineering,which has translation potentials in clinical practice.

关 键 词：HYDROGEL AGAROSE POLYDOPAMINE Cell adhesion Tissue engineering 

分 类 号：R318.08[医药卫生—生物医学工程]