响应面法优化积雪草的微波提取工艺  

Optimization of microwave-assisted extraction of Centella asiatica by response surface methodology

作　　者：徐敬朴[1] 李德强[1] 郑丽亚[1] 程新杰 卞广丽 XU Jingpu;LI Deqiang;ZHENG Liya;CHENG Xinjie;BIAN Guangli(Department of Pharmacy,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院药学部,石家庄050000

出　　处：《西北药学杂志》2021年第1期1-5,共5页Northwest Pharmaceutical Journal

基　　金：河北省中医药管理局课题(编号:2018124);河北省人才培养基金项目(编号:A201902012)。

摘　　要：目的优化积雪草中积雪草苷和羟基积雪草苷的微波提取工艺。方法以液料比、乙醇体积分数、微波功率和提取时间为自变量,积雪草苷和羟基积雪草苷的提取总量为响应值,在单因素实验的基础上,采用Box-Behnken中心组合实验,以4因素3水平的响应面法(RSM),优化积雪草的微波提取工艺条件。结果积雪草微波提取的最佳工艺条件为:液料比为10∶1,乙醇体积分数为44.55%,微波功率为300 W,提取时间为3.52 min,在此条件下,积雪草中积雪草苷和羟基积雪草苷的总提取量为33.03 mg·g^-1,与理论值33.70 mg·g^-1相对误差为2.01%。结论该方法具有高效、简便的优点,可用于积雪草中积雪草苷和羟基积雪草苷的提取。Objective To optimize the microwave-assisted extraction of asiaticoside and madecassoside from Centella asiatica.Methods Liquid-solid ratio,ethanol content,microwave power and extraction time were selected as the independent variables,meanwhile,the total yields of asiaticoside and madecassoside were studied as the response value.Based on single-factor test,the optimal extraction technology was established through Box-Behnken center composite design and the method of response surface methodology(RSM)with 4 factors and 3 levers.Results The optimal extraction conditions were obtained as follows:liquid-solid ratio of 10∶1,ethanol content of 44.55%,microwave power of 300 W and extraction time of 3.52 min.Under this conditions,the total yields of asiaticoside and madecassoside were 33.03 mg·g^-1,the relative error was 2.01%compared with the predicted value of 33.70 mg·g^-1.Conclusion The method has advantages of high efficiency and simplicity,which can be used for the extraction of asiaticoside and madecassoside from Centella asiatica.

关 键 词：积雪草 响应面法 积雪草苷 羟基积雪草苷 微波提取 

分 类 号：R282[医药卫生—中药学]