3D printing of multilayered scaffolds for rotator cuff tendon regeneration  

作　　者：Xiping Jiang Shaohua Wu Mitchell Kuss Yunfan Kong Wen Shi Philipp N.Streubel Tieshi Li Bin Duan 

机构地区：[1]Mary&Dick Holland Regenerative Medicine Program,Division of Cardiology,Department of Internal Medicine,University of Nebraska Medical Center,Omaha,NE,68198,USA [2]Molecular Genetics and Cell Biology Program,Department of Genetics,Cell Biology and Anatomy,University of Nebraska Medical Center,Omaha,NE,68198,USA [3]College of Textiles&Clothing,Collaborative Innovation Center of Marine Biomass Fibers,Qingdao University,Qingdao,266071,China [4]Department of Orthopedic Surgery and Rehabilitation,University of Nebraska Medical Center,Omaha,NE,68198,USA [5]Department of Pediatrics,University of Nebraska Medical Center,Omaha,NE,68198,USA [6]Department of Surgery,College of Medicine,University of Nebraska Medical Center,Omaha,NE,68198,USA [7]Department of Mechanical and Materials Engineering,University of Nebraska-Lincoln,Lincoln,NE,68516,USA

出　　处：《Bioactive Materials》2020年第3期636-643,共8页生物活性材料（英文）

基　　金：supported by Mary&Dick Holland Regenerative Medicine Program start-up grant and pilot grant,Nebraska Research Initiative Funding,NIH(R01 AR073225);B.D.and P.S.X.J is partially supported by the China Scholarship Council;the Electron Microscopy Core Facility(EMCF)and Tissue Science Facility(TSF)at UNMC for technical assistance.

摘　　要：Repairing massive rotator cuff tendon defects remains a challenge due to the high retear rate after surgical intervention.3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties,as well as controllable microenvironments for tendon regeneration.In this study,we developed a new strategy for rotator cuff tendon repair by combining a 3D printed scaffold of polylactic-co-glycolic acid(PLGA)with cell-laden collagen-fibrin hydrogels.We designed and fabricated two types of scaffolds:one featuring a separate layer-by-layer structure and another with a tri-layered structure as a whole.Uniaxial tensile tests showed that both types of scaffolds had improved mechanical properties compared to single-layered PLGA scaffolds.The printed scaffold with collagen-fibrin hydrogels effectively supported the growth,proliferation,and tenogenic differentiation of human adipose-derived mesenchymal stem cells.Subcutaneous implantation of the multilayered scaffolds demonstrated their excellent in vivo biocompatibility.This study demonstrates the feasibility of 3D printing multilayered scaffolds for application in rotator cuff tendon regeneration.

关 键 词：Collagen-fibrin hydrogel Tenogenic differentiation Tendon repair Tissue engineering 

分 类 号：TP391.73[自动化与计算机技术—计算机应用技术] R318[自动化与计算机技术—计算机科学与技术]