Karyological observations of Ulva linza chromosomes  

作　　者：Xiaohui ZHAO Xiaoqian YANG Jianheng ZHANG Qinlin WEN Peimin HE 

机构地区：[1]College of Marine Ecology and Environment,Shanghai Ocean University,Shanghai 201306,China [2]Water Environment&Engineering Research Center of Shanghai Institution of Higher Education,Shanghai 201306,China

出　　处：《Journal of Oceanology and Limnology》2021年第1期259-265,共7页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(No.2018YFD0901500);the Shanghai Sailing Program(No.17YF1407900),the National Natural Science Foundation of China(No.41576163);the Ocean Public Welfare Scientifi c Research Project,China(No.201205010)。

摘　　要：Abstract Ulva linza is one of the species that causes green tides in the Yellow Sea,China.Due to the diffi culties in chromosomal preparation,the large numbers of chromosomes,and their relatively small sizes,there have been no reported studies on Ulva macroalgae chromosomes.The karyotypes and chromosomes in U.linza were observed after a series of treatments.The chromosomes were pretreated with 0.1%colchicine for 12 h and then mixed with enzymes.The samples were dropped from 30 cm height onto glass slides,which spread out the surface coat.These pretreatments were the optimal chromosomes preparation treatments.The prepared chromosomes were stained with 4',6-diamidino-2-phenylindole(DAPI),which is a fl uorescent probe sensitive and specifi c to DNA.The chromosome number in the haploid male and female gametophytes was n=9,and was 2 n=18 in the diploid sporophytes.The female gametophyte chromosomes were between 0.804 and 2.292μm in size,the male gametophyte chromosomes were between 0.917 and 2.916μm,and the sporophyte chromosomes were between 0.912 and 2.167μm.The relative sizes of the chromosomes were used to analyze the karyotypes of the female and male gametophyte chromosomes.The results provide a solid foundation for the basic technique that can be used to localize molecular markers of Ulva chromosomes.

关 键 词：Ulva linza GAMETOPHYTES SPOROPHYTES CHROMOSOME KARYOTYPE 

分 类 号：Q943[生物学—植物学]