Eff ects of temperature on photosynthetic performance and nitrate reductase activity in vivo assay in Gracilariopsis lemaneiformis (Rhodophyta)  

作　　者：Zhihai ZHONG Zhengyi LIU Longchuan ZHUANG Wanlin SONG Weizhou CHEN 

机构地区：[1]Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China [2]Shantou University,Shantou 515063,China

出　　处：《Journal of Oceanology and Limnology》2021年第1期362-371,共10页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(No.2017YFC0506200);the Shandong Province Science Foundation for Youths(No.ZR201807120023);the Key Research and Development Program of Yantai(No.2018ZHGY082);the China Agriculture Research System(No.CARS-50);the Financial Fund of the Ministry of Agriculture and Rural Aff airs,China(No.NFZX2018);the Project of Guangdong Province Education Department(No.2017KCXTD014)。

摘　　要：Gracilariopsis lemaneiformis is an economically-valued species and widely cultured in China at present.After being acclimated to diff erent growth temperatures(15,20,25,and 30°C)for 7 days,the relative growth rate(RGR),nitrate reductase activity,soluble protein content and chlorophyll a fl uorescence of G.lemaneiformis were examined.Results show that RGR was markedly aff ected by temperature especially at 20°C at which G.lemaneiformis exhibited the highest eff ective quantum yield of PSII[Y(II)]and lightsaturated electron transport rate(ETR max),but the lowest non-photochemical quenching.Irrespective of growth temperature,the nitrate reductase activity increased with the incubation temperature from 15 to 30°C.In addition,the greatest nitrate reductase activity was found in the thalli grown at 20°C.The value of temperature coeffi cient Q10 of alga cultured in 15°C was the greatest among those of other temperatures tested.Results indicate that the optimum temperature for nitrate reductase synthesis was relatively lower than that for nitrate reductase activity,and the relationship among growth,photosynthesis,and nitrate reductase activity showed that the optimum temperature for activity of nitrate reductase in vivo assay should be the same to the optimal growth temperature.

关 键 词：chlorophyll a fl uorescence Gracilariopsis lemaneiformis GROWTH nitrate reductase activity 

分 类 号：S917.3[农业科学—水产科学]