Inhibition of bacterial adhesion and biofilm formation by a textured fluorinated alkoxyphosphazene surface  

作　　者：Meixian Tang Chen Chen Jieru Zhu Harry R.Allcock Christopher A.Siedlecki Li-Chong Xu 

机构地区：[1]Department of Biomedical Engineering,The Pennsylvania State University,University Park,PA,16802,United States [2]Department of Chemistry,The Pennsylvania State University,University Park,PA,16802,United States [3]Department of Surgery,The Pennsylvania State University,College of Medicine,Hershey,PA,17033,United States

出　　处：《Bioactive Materials》2021年第2期447-459,共13页生物活性材料（英文）

基　　金：Research reported in this publication was supported by the National Institute of Allergy and Infectious Disease of NIH under award number R21 AI139706.

摘　　要：The utilization of biomaterials in implanted blood-contacting medical devices often induces a persistent problem of microbial infection,which results from bacterial adhesion and biofilm formation on the surface of biomaterials.In this research,we developed new fluorinated alkoxyphosphazene materials,specifically poly[bis(octafluoropentoxy)phosphazene](OFP)and crosslinkable OFP(X–OFP),with improved mechanical properties,and further modified the surface topography with ordered pillars to improve the antibacterial properties.Three X–OFP materials,X–OFP3.3,X–OFP8.1,X–OFP13.6,with different crosslinking densities were synthesized,and textured films with patterns of 500/500/600 nm(diameter/spacing/height)were fabricated via a two stage soft lithography molding process.Experiments with 3 bacterial strains:Staphylococcal epidermidis,Staphylococcal aureus,and Pseudomonas aeruginosa showed that bacterial adhesion coefficients were significantly lower on OFP and X–OFP smooth surfaces than on the polyurethane biomaterial,and surface texturing further reduced bacterial adhesion due to the reduction in accessible surface contact area.Furthermore the anti-bacterial adhesion effect shows a positive relationship with the crosslinking degree.Biofilm formation on the substrates was examined using a CDC biofilm reactor for 7 days and no biofilm formation was observed on textured X–OFP biomaterials.The results suggested that the combination of fluorocarbon chemistry and submicron topography modification in textured X–OFP materials may provide a practical approach to improve the biocompatibility of current biomaterials with significant reduction in risk of pathogenic infection.

关 键 词：Fluorinated polyphosphazene Textured surface CROSSLINKING Anti-bacterial adhesion Anti-biofilms Microbial infection 

分 类 号：R318[医药卫生—生物医学工程]