Prevalence of fumonisin producing Fusarium verticillioides associated with cereals grown in Karnataka(India)  

作　　者：N.Deepa H.Nagaraja M.Y.Sreenivasa 

机构地区：[1]Department of Studies in Microbiology,University of Mysore,Manasagangotri,Mysore 570006,Karnataka,India

出　　处：《Food Science and Human Wellness》2016年第3期156-162,共7页食品科学与人类健康（英文）

基　　金：We thank the Department of Science and Technology(DST-SERB)India,for providing grants through Young Scientist FAST TRAK research project(No.SR/FT/LS-176/2009;30.04.2012)。

摘　　要：total of 135 cereal samples were collected from different districts of Karnataka state,India in which 69 samples were infected with Fusarium species.Amongthese 51 samples were having Fusarium verticillioides infection and among them 42 samples were positive for fumonisin production.Per cent incidence and frequency were high in maize samples with 33.12%and 47.54%,respectively followed by paddy and sorghum,while pearl millet was free from F.verticillioides infection.Relative density of F.verticillioides association was 59.50%among the screened samples.A total of 326 Fusarium species were isolated by screening 135 cereal samples and among these 194 isolates of F.verticillioides scored positive for VERTF-1 and VERTR species-specific pair of primers.Further amplification with VERTF-1 and VERTF-2 pair of primers recorded 176 isolates of fumonisin producingF.verticillioides.The study revealed high incidence,frequency and relative density of fumonisin producingF.verticillioides and production of fumonisins in cereals.Itwas amplified using one forward and two reverse primers to discriminate fumonisin producing from fumonisin non-producing F.verticillioides which stresses the need for the development of managemental stratergies before they enter into the food chain.

关 键 词：CEREAL PADDY FUSARIUM 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]