Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry  

作　　者：Junmei Ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li 

机构地区：[1]Hebei Key Laboratory of Forensic Medicine,College of Forensic Medicine,Hebei Medical University,Shijiazhuang 050017,China [2]Hebei Food Safety Key Laboratory,Hebei Food Inspection and Research Institute,Shijiazhuang 050227,China

出　　处：《Food Science and Human Wellness》2020年第4期363-369,共7页食品科学与人类健康（英文）

基　　金：This work was supported by“National Key Research and Development Program of China”(Project No.2018YFC1603400);Science and Technology Program of Hebei Province(Project No.19225503D).

摘　　要：The aim of this work was to develop an automated on-line solid phase extraction(SPE)with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish samples.Samples were extracted with 0.2%formic acid acetonitrile solution,purified by on-line SPE device with HLB column,then separated by XBridge C18 column,using 0.1%formic acid solution and acetonitrile as the mobile phase.Mass spectrometric data was acquired under multiple reaction monitoring(MRM)mode using positive ionization electrospray.Internal standard method was used in the quantification,good linear relationship was got in range of 0.1–100 ng/mL and correlation coefficient was higher than 0.9990.The limits of detection were in the range of 0.125–2.00g/kg and the limits of quantitation were in the range of 0.250–5.00g/kg.Recoveries of the method were in range of 78.3%–99.3%,relative standard deviation were lower than 10%.The method was simple,sensitivity,and could be used for routine supervision and analysis of fifteen sulfonamides in pork and fish.

关 键 词：Liquid chromatography–tandem mass SPECTROMETRY On-line solid phase extraction SULFONAMIDES Internal standard quantification 

分 类 号：O657.63[理学—分析化学] TS251.7[理学—化学] TS254.7[轻工技术与工程—农产品加工及贮藏工程]