Study on prebiotic effectiveness of neutral garlic fructan in vitro  

作　　者：Ning Zhang Xuesong Huang Yanhua Zeng Xiyang Wu Xichun Peng 

机构地区：[1]Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China

出　　处：《Food Science and Human Wellness》2013年第3期119-123,共5页食品科学与人类健康（英文）

基　　金：We are grateful to the National Natural Science Foundation of China for financial support(No.31171722).

摘　　要：Garlic is traditionally believed to have many health benefits including prevention of gastrointestinal diseases.One of the major components,garlic fructan(GF),was evaluated for its prebiotic effectiveness on human intestinal microflora.Garlic fructans A(DP 16)and B(DP 21)were prepared by ethanol fractionation precipitation.Then,they were added to an in vitro fermentation system as the sole carbon source inoculated with human fecal suspension.The total anaerobic bacteria,Bacteroides and Bifidobacteria,were enumerated by plate counting on selective media.Terminal restriction fragment length polymerization(tRFLP)was used to analyze DNA extracted from the in vitro cultures.The results indicated that the log CFUs of both Bacteroides(GF A 6.96,GF B 7.15)and Bifidobacteria(GF A 7.74,GF B 7.74)grown in the GF cultures at 24 h were significantly higher than those at 0 h(Bacteroides 4.93,Bifidobacteria 4.78)(P<0.05).Terminal restriction fragments(TRFs)256–258 bp(ascribed to Bifidobacterium)in the profiles were also observed higher in the TRFLP profiles from the garlic fructans’cultures.In this study,GFs were found to selectively stimulate the growth of beneficial Bifidobacteria from human fecal microflora.The prebiotic effectiveness of GFs supports the use of garlic as a way to prevent some gastrointestinal diseases.©2013 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.

关 键 词：Garlic fructans Prebiotic effectiveness In vitro fermentation Fecal bacteria 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]