矮壮素处理促进葡萄二季果成花的分子机制研究  

Study on the molecular mechanism of promotion of inflorescence formation of CCC treatment for the second fruiting in grape

作　　者：时晓芳 韦荣福 黄桂媛 张瑛 林玲 韩佳宇 曹雄军 周思泓 王博[2] 白先进[3] 郭荣荣 SHI Xiaofang;WEI Rongfu;HUANG Guiyuan;ZHANG Ying;LIN Ling;HAN Jiayu;CAO Xiongjun;ZHOU Sihong;WANG Bo;BAI Xianjin;GUO Rongrong(Grape and Wine Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;College of Agriculture,Guangxi University,Nanning 530007,Guangxi,China;Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China)

机构地区：[1]广西壮族自治区农业科学院葡萄与葡萄酒研究所,南宁530007 [2]广西大学农学院,南宁530007 [3]广西壮族自治区农业科学院,南宁530007

出　　处：《果树学报》2021年第2期153-167,共15页Journal of Fruit Science

基　　金：国家自然科学基金(31460501);广西自然科学基金(2017GXNSFBA198100);广西重点研发计划(桂科AB18126005);广西农业科学院稳定资助科研团队项目(桂农科2021YT127)。

摘　　要：【目的】探究矮壮素处理后'夏黑'葡萄二季果成花的主要生物学过程和重要基因,为促进二季果成花提供理论依据。【方法】以矮壮素处理后'夏黑'葡萄二季果第5~6节位冬芽为试验材料,采用转录组测序技术筛选差异表达基因(DEGs),并对其进行分析。【结果】与对照相比,矮壮素处理后-3 DAF、4 DAF、11 DAF冬芽样品中差异基因数分别为395、557和97个。其中-3 DAF、4 DAF、11 DAF冬芽样品中分别有52、21、39个DEGs上调表达,343、536、58个DEGs下调表达,共有879个DEGs得到注释,富集最显著的Pathway主要有苯丙素和类黄酮生物合成、激素信号转导等。【结论】通过转录组测序和qRT-PCR验证,发现矮壮素处理'夏黑'葡萄后,二季果成花关键节位冬芽内苯丙素生物合成途径中的POD47和PODN1、类黄酮生物合成途径中的STS6、生长素信号转导通路中的IAA26、赤霉素信号转导通路中的GARP6和GA3OX1等基因的表达量发生明显变化,说明矮壮素促进成花的功能可能通过改变树体内激素的含量及比值、次级代谢产物的合成和代谢来实现。【Objective】The study aimed to explore the main biological processes and important genes involved in inflorescence formation for the second fruiting of’Summer Black’grape after chlormequat chloride(CCC)treatment through screening the differential expression genes(DEGs)in the key node buds for the second fruiting at different development stages by transcriptome sequencing.【Methods】Leaves of the’Summer Black’grape were sprayed with 0.5 g·L^-1 CCC on the 10 th and 1 st day before full blooming.The control plants were sprayed with purified water.In the control and CCC treatment,when the 14 th leaf of the main shoot unfolded,the top of the shoot was removed and all the auxiliary shoots were wiped out.The latent buds on the 5 th and 6 th nodes were used as experimental materials,and these buds were collected on the 3 rd day before full blooming,4 th day after full blooming(DAF)and 11 th DAF of the first season fruit of’Summer Black’grape,respectively.The samplings were carried out on 7 th,14 th and 21 th day after the first CCC treatment.The total RNA of latent buds was extracted,and the genomic DNA was removed.The differences in transcript abundance values among the samples were calculated using the ratio of Fragments Per Kilobase of transcript per million mapped reads(FPKM),and the significance of the differences was computed using the false discovery rate(FDR).Genes with a log2 ratio≥2 and FDR significance score<0.01 were regarded as DEGs.Gene Ontology(GO)function enrichment and KEGG pathway enrichment analysis of DEGs were performed using Plant Met Gen MAP.The K-means clustering were performed using Gene-E to reveal the expression patterns of the DEGs at the three time points.【Results】The number of DEGs on the 3 rd DAF,4 th DAF and 11 th DAF post CCC treatment was 395,557 and 97,respectively,compared with the control,among them 52,21 and 39 genes were up regulated and 343,536 and 58 genes were down regulated,respectively.GO analysis results showed that in the process of inflorescence primo

关 键 词：葡萄 二季果 成花 矮壮素 分子机制 

分 类 号：S663.1[农业科学—果树学]