PM_(2.5)对HK-2肾细胞部分癌基因和凋亡基因表达的影响  

Effects of PM_(2.5) exposure on expression of oncogenes and apoptosis genes in human renal epithelial cells

作　　者：李柏茹 秦双建 李闰冰 蔡颖[2,3] 郑凯[2,3] 王冰玉 曾明 肖芳[1] 徐新云[2] LI Boru;QIN Shuangjian;LI Runbing;CAI Ying;ZHENG Kai;WANG Bingyu;ZENG Ming;XIAO Fang;XU Xinyun(Xiangya School of Public Health,Central South University,Changsha 410078,Hunan;Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,Guangdong;School of Public Health,University of South China,Hengyang 421001,Hunan,China)

机构地区：[1]中南大学湘雅公共卫生学院,湖南长沙410078 [2]深圳市疾病预防控制中心,广东深圳518055 [3]南华大学公共卫生学院,湖南衡阳421001

出　　处：《癌变．畸变．突变》2021年第1期6-11,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：深圳市科技研发项目(JCYJ20170413101713324,JCYJ20190807102205480)。

摘　　要：目的:探讨PM_(2.5)对人肾上皮细胞(HK-2)部分癌基因和凋亡基因表达的影响。方法:利用CCK-8试剂盒测定PM_(2.5)混悬液对HK-2细胞的半数抑制浓度(IC50);实验设阴性对照组、PM_(2.5)混悬液低剂量(10μg/mL)、高剂量(50μg/mL)染毒组和阳性对照组(Cr6+10μmol/L),分别对HK-2细胞处理24 h后利用实时荧光定量PCR(qPCR)和Western blot检测癌基因(c-myc、c-fos、p53)和凋亡基因(Caspase-8、Caspase-9、Bcl-2)mRNA和蛋白的表达水平。结果:PM_(2.5)混悬液对HK-2细胞的IC50为95.98μg/mL;经PM_(2.5)混悬液染毒24 h,qPCR结果显示,与阴性对照组比较,PM_(2.5)混悬液高剂量组和阳性对照组c-myc、c-fos、Caspase-8、Caspase-9 mRNA表达均升高,差异均有统计学意义(P<0.01);p53和Bcl-2 mRNA表达降低,差异均有统计学意义(P<0.01)。Western blot结果显示,与阴性对照组比较,PM_(2.5)混悬液高剂量组和阳性对照组c-myc、c-fos、Caspase-8、Caspase-9蛋白表达均升高,差异均有统计学意义(P<0.01);p53和Bcl-2蛋白表达均降低,差异均具有统计学意义(P<0.05)。结论:PM_(2.5)可引起HK-2细胞中部分癌基因表达上升、抑癌基因表达下降、促凋亡基因表达上升和抑凋亡基因表达下降,提示PM_(2.5)对HK-2肾细胞部分癌基因和凋亡基因具有一定的促进与激活作用。OBJECTIVE:To investigate the effect of PM_(2.5) exposure on expression of oncogenes and apoptosis genes in human renal epithelial cells(HK-2).METHODS:The CCK8 assay was used to obtain the half maximal inhibitory concentration(IC50)of Taiyuan PM_(2.5) on human renal epithelial(HK-2)cells.HK-2 cells were treated with PM_(2.5) for 24 h.Four groups were set up:negative control group,low-dose PM_(2.5) group(10μg/mL PM_(2.5)),high-dose PM_(2.5) group(50μg/mL PM_(2.5))and positive control group(Cr6+10μmol/l).qPCR and Western blot were used to detect mRNA and proteins levels of apoptosis genes and oncogenes(c-myc,cfos,p53,Caspase-8,Caspase-9 and Bcl-2).RESULTS:The IC50 value of Taiyuan PM_(2.5) on HK-2 cells was 95.98μg/mL.After 24 hours of PM_(2.5) exposure,qPCR results show that,compared with the control group,expressions of c-myc,c-fos,Caspase-8,Caspase-9 mRNAs were significantly increased in the high-dose and positive control groups(P<0.01).However,expressions of p53 and Bcl-2 mRNAs were significantly decreased in these two groups(P<0.01).Western blot data show that,compared with the control group,expressions of cmyc,c-fos,Caspase-8,Caspase-9 proteins were significantly increased in the high-dose and positive control groups(P<0.01).However,expressions of p53 and Bcl-2 proteins were significantly decreased in these two groups(P<0.05).CONCLUSION:PM_(2.5) exposure to HK-2 cells increased the expression of oncogenes and apoptosis genes but decreased the expression of tumor suppressor and anti-apoptosis genes.Our results suggest that PM_(2.5) exposure can promote oncogenesis.

关 键 词：大气细颗粒物 HK-2细胞 癌基因 凋亡基因 

分 类 号：R122.7[医药卫生—环境卫生学]