不同材料压舌板体外细胞毒性效应研究  

In vitro cytotoxicity evaluation of tongue depressors with different materials

作　　者：罗吉 陈小青 李冰 马中春 LUO Ji;CHEN Xiaoqing;LI Bing;MA Zhongchun(Ningbo Customs District Technology Center,Ningbo 315100,Zhejiang,China)

机构地区：[1]宁波海关技术中心,浙江宁波315100

出　　处：《癌变．畸变．突变》2021年第1期58-61,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：海关总署科研项目(2019HK072);宁波国家高新区(新材料科技城)重大技术创新项目(20181CX050016)。

摘　　要：目的:对不同材料压舌板的体外细胞毒性效应进行评价,旨在为压舌板的安全使用提供科学依据。方法:将3种材料的压舌板分别配制成25%、50%、75%、100%等不同浓度的浸提液作为受试物组,另设空白对照组、阴性对照组和阳性对照组,采用L929小鼠成纤维细胞株体外培养24 h,置于显微镜下观察细胞形态,通过MTT法检测受试物组和对照组的吸光度值进行定量分析,对压舌板的体外细胞毒性效应进行综合评价。结果:空白对照组和阴性对照组胞内颗粒明显,细胞无溶解,生长状态良好。阳性对照组所有细胞坏死。100%木制压舌板浸提液组几乎所有细胞全部坏死,100%竹制压舌板浸提液组胞内颗粒明显,细胞无溶解,生长状态良好,100%不锈钢压舌板浸提液组圆缩、疏松贴壁及无胞内颗粒的细胞比例小于20%。经MTT法所测D(570)值计算得出100%木制压舌板浸提液的细胞存活率为20.1%;100%竹制压舌板浸提液的细胞存活率为89.1%;100%不锈钢压舌板浸提液的细胞存活率为81.2%。根据细胞毒性判定标准,木制压舌板对L929细胞有潜在细胞毒性。结论:木制压舌板具有潜在细胞毒性,在使用过程中应注意加强安全保障。OBJECTIVE:To evaluate the in vitro cytotoxic effects of tongue depressors with different materials and their scientific bases for toxicity.METHODS:The L929 cell line was cultured and organized into different groups:blank control,negative control,positive control and treatment groups.The latter group was treated with tongue depressors of the three materials with 25%,50%,75%and 100%extraction concentrations.After 24 h of treatment,all cell cultures were evaluated for their morphology under a microscope,viability using the MTT method and other cytotoxic effects.RESULTS:The results showed that cells in the positive control group and in the group which was treated with the 100%extract from wooden tongue depressors were completely destroyed.On the other hand,cells in the group which was treated with 100%extract from bamboo tongue depressor grew normally.Approximately 20%of the cells in the group which was treated with 100%extract from stainless steel tongue depressor showed abnormal morphologies:round with shrinkage,poor adherence to the wall and lacking intracellular particles.Results from the MTT assay show that the cell viability for the mentioned two groups which indicated toxicity were 89.1%(bamboo)and 81.2%(stainless steel).According to criteria of cytotoxicity,exposure to extracts from wooden tongue depressors caused potential cytotoxicity.CONCLUSION:Our results indicate that tongue depressors which were made of certain materials caused cytotoxic effects in vitro.Our data should be useful in strengthening safety guidelines for use of these tongue depressors.

关 键 词：压舌板 L929细胞 细胞毒性 噻唑蓝 

分 类 号：R114[医药卫生—卫生毒理学]