烟草自然抗性相关巨噬细胞蛋白基因家族的鉴定与分析  

Identification and Analysis of Natural Resistance Associated Macrophage Proteins Gene Family in Tabacco

作　　者：陈邦兰 翟梦倩 龙涛 赵顺程 刘娟 尹茂灵 万心言 王治杰 刘继恺[1,3] CHEN Bang-lan;ZHAI Meng-qian;LONG Tao(College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010;College of Biology and Geography,Yili Normal University,Yining,Xinjiang 835000)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]伊犁师范大学生物与地理科学学院,新疆伊宁835000 [3]西南科技大学核废物与环境安全国防重点实验室,四川绵阳621010

出　　处：《安徽农业科学》2021年第3期100-104,109,共6页Journal of Anhui Agricultural Sciences

基　　金：西南科技大学大学生创新创业训练项目(S201910619065);西南科技大学龙山人才科研支持计划(18LZX626)。

摘　　要：自然抗性相关巨噬细胞蛋白(natural resistance associated macrophage proteins,NRAMPs)是一类高度保守的二价金属跨膜转运蛋白,在调控生物体内重金属稳态中发挥着重要作用。为了探究烟草NRAMP基因的多样性,该研究根据烟草基因组数据信息,利用生物信息学方法对烟草NRAMP基因家族成员进行了鉴定,对其基本信息、结构特点、蛋白理化性质及保守序列、启动子元件和组织表达模式等进行了分析。结果表明:烟草基因组含有9个NtNRAMP基因,分别命名为NtNRAMP1.1~6.2,其编码氨基酸长度为500~542 aa,蛋白分子量为54.31~59.11 kD,等电点为5.09~8.73,总平均亲水性为0.475~0.604;除了NRAMP6.1和6.2不含有motif4外,其余NRAMP蛋白都含有motif1~motif10。顺式作用元件分析表明所有NtNRAMP基因启动子中均含有参与基因转录、非生物胁迫、植物激素响应和光应答途径的元件,部分基因含有生物胁迫、次生代谢、组织表达和结合位点的元件。染色体定位发现,6个NtNRAMP基因分别分布在5条烟草染色体上。表达分析表明:NtNRAMP基因组织表达模式差异较大,其中NtNRAMP6.1和NtNRAMP6.2基因表达具有组织特异性。该研究结果为进一步研究烟草NtNRAMP基因的功能奠定了基础。Natural resistance associated macrophage proteins(NRAMPs)are highly conserved bivalent metal transporters that play an important role in regulating heavy metal homeostasis.In order to explore the diversity of the NRAMP gene in tobacco,this study identified the members of the NRAMP gene family of tobacco by using bioinformatics methods based on the genome data of N.tabacum,and analyzed the basic information,structural features,physicochemical parameters,conserved motifs,cis-acting elements,tissue expression pattern of these NtNRAMP genes.The results showed that N.tabacum genome contained 9 NtNRAMP genes which were designated as NtNRAMP1.1-6.2.The length of NtNRAMP proteins ranged from 500 to 542 amino acid;the relative molecular weight ranged from 54.31 to 59.11 kD;the predicted pI ranged from 5.09 to 8.73,and the predicted GRAVY ranged from 0.475 to 0.604.Except for NRAMP6.1 and NRAMP 6.2,which did not contained motif4,all NtNRAMP proteins contained motif1-motif10.Cis-acting regulatory element prediction showed that all NtNRAMP genes contained elements involved in gene transcription,abiotic stress,plant hormone response and light response pathways,however,some genes contained elements involved in biological stress,secondary metabolism,tissue expression and binding sites.Chromosomal localization revealed that 6 of 9 NtNRAMP genes were located in 5 tobacco chromosomes.The tissue expression profile analysis showed diverse tissue expression patterns of NtNRAMPs,and NtNRAMP6.1 and NtNRAMP6.2 showed tissue specific expression patterns.These results laid a foundation for further functional characterization of NtNRAMP genes.

关 键 词：自然抗性相关巨噬细胞蛋白 烟草 基因家族 表达分析 

分 类 号：S572[农业科学—烟草工业]