天门冬质量及RAPD遗传多样性研究  

Study on Quality and RAPD Genetic Diversity of Asparagi Radix

作　　者：徐昌艳[1] 罗忠圣 曹旭林 钱志瑶 李相陵[1] 覃容贵[1] XU Chang-yan;LUO Zhong-sheng;CAO Xu-lin(Guizhou Medical University,Guiyang,Guizhou 550025;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang,Guizhou 550014;Guiyang Sixth Hospital,Guiyang,Guizhou 550005)

机构地区：[1]贵州医科大学,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014 [3]贵阳市第六人民医院,贵州贵阳550005

出　　处：《安徽农业科学》2021年第3期180-184,共5页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技厅科技合作计划项目(黔科合LH字〔2015〕7329);2017年国家级大学生创新创业训练计划项目(DC201710660019);2019年国家级大学生创新创业训练计划项目(201910660028)。

摘　　要：[目的]研究天门冬质量及RAPD遗传多样性,并探索其质量与RAPD遗传多样性间相关性。[方法]采用综合评分法,以总皂苷、多糖、浸出物含量为评价指标对天门冬进行质量评价;对天门冬RAPD反应体系进行优化,完成不同居群天门冬RAPD扩增,运用NTSYS2.10e软件进行遗传多样性分析及UPGMA系统树图构建;分析天门冬质量与RAPD遗传多样性的相关性。[结果]18份天门冬样品质量差异较大;18份天门冬多态性百分率为89.62%,遗传相似系数为0.4481~0.7486,在阈值0.52处18份天门冬聚为两大类,其中贵州遵义桐梓天门冬单独分为一类,其余聚为一类。[结论]天门冬质量与RAPD遗传多样性无显著相关性,所建立的天门冬RAPD法可用于其遗传多样性研究,可为其良种选育、规范化种植奠定基础。[Objective]The quality and RAPD genetic diversity of Asparagi Radix were studied,and the correlation between the quality and RAPD genetic diversity of Asparagi Radix was explored.[Method]The content of the total saponins,polysaccharide and alcohol-soluble extract in Asparagi Radix was taken as the evaluation index,and the quality of Asparagi Radix was evaluated by the comprehensive scoring method.We optimized the RAPD reaction system of Asparagi Radix,and amplified different populations of Asparagi Radix,based on the RAPD band data,the genetic similarity coefficients were analysed by NTSYS 2.10e,and the UPGMA dendrogram were developed.The correlation between the genetic diversity of RAPD and the quality of Asparagi Radix was analyzed.[Result]The quality of 18 Asparagi Radix samples was very different.The polymorphism rate of 18 Asparagi Radix was 89.62%,and the genetic similarity coefficient was 0.4481-0.7486.In the UPGMA analysis,under the threshold of 0.52,18 samples were divided into two groups,1 sample from Zunyi County was clustered into one group,while the rest of the 17 samples were clustered into the other group.[Conclusion]There is no significant correlation between the quality and genetic diversity of Asparagi Radix.The established RAPD method of Asparagi Radix can be used for the study of its genetic diversity,which can lay the foundation for the breeding of improved varieties and standardized planting of Asparagi Radix.

关 键 词：天门冬 质量评价 RAPD 遗传多样性 UPGMA 

分 类 号：R282.71[医药卫生—中药学]