番泻的不同部位中番泻苷A、B的含量研究  

Study on the Content of A and B in Different Parts of Senna

作　　者：谭琪明[1] 何珺[2] 李满香 马海霞 Tan Qiming;He Jun;Li Manxiang;Ma Haixia(Tongren Vocational and Technical College,Tongren 554300,China;Guizhou Biochemical Engineering Center,Guiyang 550025,China;Guizhou Agricultural Vocational College,Qingzhen 551400,China)

机构地区：[1]铜仁职业技术学院,贵州铜仁554300 [2]贵州省生化工程中心,贵州贵阳550025 [3]贵州农业职业学院,贵州清镇551400

出　　处：《山东化工》2021年第1期112-114,共3页Shandong Chemical Industry

基　　金：铜仁市科技计划项目[课题任务书合同编号:铜市科研(2017)47-101号]。

摘　　要：通过方法学考察,确定了简便、快速的番泻苷A、B HPLC含量检测法,其条件为Supersil-T C18柱,流动相为乙腈-0.4%磷酸水,其检测的波长为340 nm,柱温在25℃;流速为1.0 mL·min^-1;进样量是10μL。从研究我们可以发现,番泻叶以及番泻豆荚等不同部位中番泻苷A、B的含量得出,番泻豆荚中番泻苷A和B中的总含量要比番泻叶高,而且在不同部位含量也是不一样的:豆荚壳>叶片≈叶柄>果柄>种子。A simple and rapid HPLC method for the detection of Senatine A and B was established based on supersil-T C18 column and acetonitrile-0.4% phosphoric acid water.The detection wavelength was 340 nm and the temperature was 25℃.The flow rate was 1.0 mL·min^-1.The injection quantity is 10μL.It can be found from the study that the content of senna A and B in senna leaf and senna pod is higher than that in senna leaf,and the content is different in different parts.

关 键 词：番泻 番泻苷A 番泻苷B 

分 类 号：O657.7[理学—分析化学]