新疆北部地区蜱传嗜吞噬细胞无浆体病原学检测与分子遗传进化分析  

Molecular Detection and Phylogenetic Analysis of Tick-borne A.phagocytophilum in Northern Part of Xinjiang

作　　者：吴泽功 林汉亮 罗金[2] 马力克•艾则孜 任巧云[2] 罗毅 马站 殷宏[2,4] 李奎 刘光远[2,4] WU Ze-gong;LIN Han-liang;LUO Jin;MALIKE•AIZ;REN Qiao-yun;LUO Yi;MA Zhan;YIN Hong;LI Kui;LIU Guang-yuan(Henan Agricultural University,Zhengzhou,Henan,450002,China;State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,CAAS,Lanzhou,Gansu,730046,China;Animal Health Supervision Institution of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang,830011,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu,225009,China)

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]中国农业科学院兰州兽医研究所/家畜动物疫病国家重点实验室,甘肃兰州730046 [3]新疆维吾尔自治区动物卫生监督所,新疆乌鲁木齐830011 [4]江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《动物医学进展》2021年第2期19-25,共7页Progress In Veterinary Medicine

基　　金：中国农业科学院基本科研业务费专项院级统筹项目(Y2018PT76);“十三五”国家重点研发计划项目(2019YFC1200502);国家寄生虫资源库项目(NPRC-2019-194-30)。

摘　　要：为调查我国新疆北部地区蜱及牛、羊体内蜱传播性嗜吞噬细胞无浆体(Anaplasma phagocytophilum)病原的流行情况,收集了新疆北部地区13个县(市)的蜱虫样品及血液样品(蜱虫样品609只,牛血液样品670份,羊血液样品631份),提取基因组DNA,PCR检测后挑选阳性样品测序(1/3),分析其感染状况及病原分子遗传特征。结果显示,蜱虫和血液样品中共检测出183份A.phagocytophilum感染阳性,牛、羊和蜱虫感染率分别为10.0%(67/670)、9.5%(60/631)和8.2%(50/609),样品总体感染率为9.3%(177/1910)。此次获得的新疆北部地区A.phagocytophilum 16S rRNA和MSP 4基因序列遗传进化分析显示,该区牛、羊和蜱体内检测到的病原基因阳性片段与GenBank已公布的中亚、中欧地区病原基因序列同源性为96.7%~99.5%,进化树分析显示与中亚、中欧多国A.phagocytophilum基因序列聚为一支。结果可为新疆北部地区蜱与蜱传播性嗜吞噬细胞无浆体病的防控提供理论依据。In order to investigate the prevalence of tick-borne zoonotic Anaplasma phagocytophilum in the northern part of Xinjiang.A total of 609 tick samples were colleted from animal from 13 counties within the the Northern part of Xinjiang.There were also 631 goat blood samples and 670 cattle blood samples colleted.All samples were used for polymerase chain reaction assay(PCR)and sequencing analysis of positive samples.And the sequence was used to analyze of genetic characteristics.A total of 183 samples have been identified for positive samples.The infection rates of cattle,sheep and ticks were 10.0%(67/670),9.5%(60/631)and 8.2%(50/609),respectively.The overall infection rate of the samples was 9.3%(177/1910).Genetic evolution analysis showed that A.phagocytophohilum in the northern Xinjiang area was the most prevalent pathogenic genotype in the pathogenic gene sequences detected in cattle,sheep and ticks in this area,which were similar to those in Central Asia and central Europe(92.7%-99.5%).Phylogenetic tree analysis showed that the gene of A.phagocytophilum was clustered together with that of Central Asia and central Europe.This study provided a theoretical basis for the prevention and control of ticks and tick-borne anaplasmosis in Northern Xinjiang.

关 键 词：蜱 人畜共患病原 嗜吞噬细胞无浆体 病原学检测 遗传进化分析 

分 类 号：S852.71[农业科学—基础兽医学] S852.64[农业科学—兽医学]