2019年福建某原种猪场猪繁殖与呼吸综合征病毒流行毒株ORF5基因的遗传变异分析  

Genetic Variation Analysis of ORF5 Gene of PRRSV Epidemic Strain in a Breeding Farm of Fujian Province 2019

作　　者：林楚云 刘建奎[2,3] 林志锋 林日丹 杨小燕 戴爱玲[2,3] LIN Chu-yun;LIU Jian-kui;LI Zhi-feng;LI Ri-dan;YANG Xiao-yan;DAI Ai-ling(College of Animal Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian,350002,China;College of Life Sciences of Longyan University,Longyan,Fujian,364000,China;Fujian Engineering Research Center for Swine Disease Control and Prevention,Longyan,Fujian,364000,China)

机构地区：[1]福建农林大学动物科学学院,福建福州350002 [2]龙岩学院生命科学学院,福建龙岩364000 [3]福建省生猪疫病防控工程技术研究中心,福建龙岩364000

出　　处：《动物医学进展》2021年第2期46-50,共5页Progress In Veterinary Medicine

基　　金：福建省科技重大专项项目(2019NZ09005);福建省科技计划引导性项目(2017N0022);上杭县奇迈科技创新基金项目(2019SHQM06)。

摘　　要：为确诊福建某原种猪场保育猪群临床暴发的疑似猪繁殖与呼吸综合征(PRRS),以及了解猪繁殖与呼吸综合征病毒(PRRSV)毒株流行情况,从该猪场采集2周龄~8周龄各周龄段保育猪临床疑似发病猪全血各5份,将各周龄段5份血样各取50μL混匀,并分别命名为2W、3W、4W、5W、6W、7W、8W。利用PRRSV ORF5基因特异性引物通过RT-PCR方法进行检测,并对阳性结果进行ORF5基因序列分析。RT-PCR结果显示,2周~4周的保育猪全血中未检出PRRSV,5周~8周的保育猪全血中检出PRRSV。ORF5基因序列分析显示,5W毒株的ORF5基因与JXA1、HuN4和TJ等HP-PRRSV代表毒株同源性较高,为95.4%~95.6%,而与NADC30、VR2332、QYYZ等毒株的同源性较低,为82.7%~87.4%;6W、7W、8W毒株的ORF5基因与NADC30、FJZ03、FJY04和CHsx1401等类NADC30 PRRSV代表毒株同源性较高,为88.9%~91.6%,而与JXA1、VR2332、QYYZ等毒株的同源性较低,为83.3%~85.6%。基于ORF5基因构建的遗传进化树显示,5W毒株属于HP-PRRSV,6W、7W、8W毒株属于类NADC30 PRRSV。结果表明,该猪场保育猪存在类NADC30和HP-PRRSV毒株混合感染。研究结果可为福建省防控PRRS提供理论依据。In order to confirm the suspected outbreak of PRRS in a breeding farm of Fujian province and to understand the prevalence of PRRSV strain,5 whole blood samples clinically suspected were collected from 2-8-week-old piglets from the farm.50μL blood samples were taken from 5 blood samples of each week and mixed well,and named as 2W,3W,4W,5W,6W,7W and 8W,and so on.The specific primers of PRRSV ORF5 gene were detect the samples by RT-PCR,and the positive results were analyzed by ORF5 gene sequences.RT-PCR results showed that no PRRSV was detected in the whole blood of 2W-4W conservation pigs,and no PRRSV was detected in the whole blood of 5W-8W conservation pigs.The ORF5 gene sequence analysis showed that the ORF5 gene of strain 5W had a high homology with HP-PRRSV representative strains such as JXA1,HuN4 and TJ(95.4%-95.6%),but a low homology with NADC30,VR2332 and QYYZ(82.7%-87.4%).The ORF5 gene of strains 6W,7W and 8W had high homology with NADC30 PRRSV,such as NADC30,FJZ03,FJY04 and CHsx1401(88.9%-91.6%),while the homology with strains JXA1,VR2332 and QYYZ(83.3%-85.6%).The genetic evolution tree constructed based on ORF5 gene showed that strain 5W belonged to HP-PRRSV,and strains 6W,7W and 8W belonged to NADC30 PRRSV.The results showed that there were mixed infection of NADC30 and HP-PRRSV strains in the conservation pigs.This study provided theoretical basis for the prevention and control of PRRS in Fujian province.

关 键 词：猪繁殖与呼吸综合征病毒 反转录-聚合酶链反应 ORF5基因 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学] S858.28[农业科学—兽医学]