数字PCR技术在动物疫病诊断中的应用进展  

Progress on Application of Digital PCR in Animal Disease Diagnosis

作　　者：郭宏伟[1] 赵绪永[1] 李华玮[1] 张震[1] 马辉[1] 郑鸣[1] GUO Hong-wei;ZHAO Xu-yong;LI Hua-wei;ZHANG Zhen;MA Hui;ZHENG Ming(Henan University of Animal Husbandry and Economy,Zhengzhou,Henan,450011,China)

机构地区：[1]河南牧业经济学院,河南郑州450011

出　　处：《动物医学进展》2021年第2期102-106,共5页Progress In Veterinary Medicine

基　　金：河南省科技攻关项目(182102110021,202102110249)。

摘　　要：数字PCR技术是一种能够进行绝对定量的核酸检测技术,与实时荧光定量PCR技术相比,该技术敏感性更高、定量不依赖于标准曲线、对反应抑制物的耐受能力也更高。目前,该技术在转基因检测、物种鉴定、疾病诊断、病原微生物鉴定和精确定量分析等领域具有较广阔的应用前景。论文对数字PCR的发展历史、原理、优缺点及在动物疫病诊断中的应用进行了综述,以期为提高动物疫病诊断能力的提供借鉴。Digital PCR is a novel nucleic acid detection technology for absolute quantification of template.Compared to real-time PCR technology,digital PCR has several advantages,such as higher sensitivity,no calibration curve required,and resistance to PCR inhibitors.At present,the digital PCR technology has a broad application prospect in transgenic detection,species identification,disease diagnosis,pathogen identification and precise quantitative analysis.In this review,we summarized the development,principles,adva-ntages and disadvantages,and application of digital PCR in animal disease diagnosis,so as to provide some references for improving the diagnostic ability of animal diseases.

关 键 词：数字聚合酶链反应 绝对定量 动物疫病诊断 实时荧光定量聚合酶链反应 

分 类 号：S854.4[农业科学—临床兽医学] S852.65[农业科学—兽医学]