芽胞杆菌的分离鉴定  

Isolation and identification of Bacillus isolates

作　　者：李贺海 王佳慧 李迎迎 邱云飞 刘军龙[1] 刘爱红[1] 王锦明[1] 关贵全[1,2] 罗建勋 殷宏[1,2] 李有全 LI He-hai;WANG Jia-hui;LI Ying-ying;QIU Yun-fei;LIU Jun-long;LIU Ai-hong;WANG Jin-ming;GUAN Gui-quan;LUO Jian-xun;YIN Hong;LI You-quan(Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences/State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou 730046,China;Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]扬州大学,江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2021年第1期74-80,共7页Chinese Veterinary Science

基　　金：国家肉牛牦牛产业技术体系项目(CARS-37);国家重点研发计划项目(2018YFD0501804,2018YFD0502305,2016-YFD0501503);中国农业科学院基本科研业务项目(Y2020XC06-2)。

摘　　要：为了确定实验室筛选的6株芽胞杆菌(Bacillus spp.)的种名,利用形态学、DNA促旋酶A亚单位基因(DNA Gyrase Subunit A,gyr A)和DNA促旋酶B亚单位基因(DNA Gyrase Subunit B,gyr B)序列的单基因系统发育分析和PCR相结合的方法进行鉴定。结果表明,6株芽胞杆菌的单菌落生长特征相似,革兰染色呈阳性,具有芽胞;以gyr A和gyr B基因构建的单基因系统发育树显示6株菌均与贝莱斯芽胞杆菌(Bacillus velezensis)归于同一分支,自展值(Bootstrap)较高;特异性PCR结果也证实它们均为贝莱斯芽胞杆菌阳性。结论,实验室分离的6株芽胞杆菌均属于贝莱斯芽胞杆菌,这为它们的深入研究奠定了基础。In order to quickly identify six Bacillus spp.isolates,their morphology,phylogenetic analysis based on DNA gyrase subunit A(gyr A)and DNA gyrase subunit B(gyr B)gene sequences,and specific polymerase chain reaction(PCR)were performed.The results showed that the colony characteristics of 6 Bacillus spp.isolates were similar,and all of them were gram-positive and spore-forming Bacillus.The phylogenetic analysis based on gyr A and gyr B gene sequences showed that six isolates belonged to the same branch as Bacillus velezensis,and the bootstrap was relatively high.The six Bacillus spp.isolates were also positive by B.velezensis species-specific primers.In conclusion,the six Bacillus spp.isolated in our laboratory belonged to B.velezensis,and this laid the foundation for the further study of them in future.

关 键 词：贝莱斯芽胞杆菌 PCR 系统发育分析 

分 类 号：S852.616[农业科学—基础兽医学]