冠状动脉性心脏病患者肠道菌群数量与血脂及同型半胱氨酸水平的相关性  

Correlation between the amount of the intestinal flora and blood lipid,homocysteine levels in patients with coronary heart disease

作　　者：石翠 袁宇[1] SHI Cui;YUAN Yu(Department ofCardiology,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China)

机构地区：[1]新乡医学院第一附属医院心血管内科,河南卫辉453100

出　　处：《新乡医学院学报》2021年第1期44-47,共4页Journal of Xinxiang Medical University

基　　金：新乡市科技攻关计划项目(编号:CXGG16012)。

摘　　要：目的探讨冠状动脉性心脏病(CHD)患者肠道乳酸菌、双歧杆菌及大肠杆菌数量与血脂、同型半胱氨酸(HCY)水平的相关性。方法选择2019年5月至2019年8月新乡医学院第一附属医院收治的50例CHD患者为观察对象(CHD组),另选择同期健康体检者30例为对照组。于入院次日清晨抽取2组受试者肘静脉血5mL,使用全自动生物化学分析仪检测血清中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)及HCY的水平。于治疗前收集2组受试者新鲜粪便样本5.0g,使用细菌RNA提取试剂盒提取细菌总RNA,采用实时荧光定量聚合酶链反应法检测乳酸菌、双歧杆菌及大肠杆菌数量。对肠道菌群数量与血清TC、TG、HDL、LDL及HCY水平相关性进行分析。结果与对照组比较,CHD组受试者血清TC、TG、LDL、HCY水平均显著升高,HDL水平显著降低(P<0.05)。与对照组比较,CHD组受试者粪便中双歧杆菌和乳酸菌数量显著降低,大肠杆菌数量显著升高(P<0.05)。2组受试者血清TC水平与双歧杆菌数量呈负相关(P<0.05),与乳酸杆菌及大肠杆菌数量无相关性(P>0.05);血清TG水平与双歧杆菌及大肠杆菌数量呈正相关(P<0.05),与乳酸杆菌数量无相关性(P>0.05);血清HDL水平与双歧杆菌及乳酸杆菌数量呈正相关(P<0.05),与大肠杆菌数量呈负相关(P<0.05)。对照组受试者血清LDL水平与双歧杆菌数量呈正相关(P<0.05),CHD组受试者血清LDL水平与双歧杆菌数量无相关性(P>0.05);对照组受试者血清LDL水平与乳酸杆菌数量无相关性(P>0.05),CHD组受试者血清LDL水平与乳酸杆菌数量呈正相关(P<0.05);对照组受试者血清LDL水平与大肠杆菌数量呈负相关(P<0.05),CHD组受试者血清LDL水平与大肠杆菌数量无相关性(P>0.05)。2组受试者血清HCY水平与双歧杆菌数量均呈负相关(P<0.05),与大肠杆菌数量均呈正相关(P<0.05),与乳酸杆菌数量无相关性(P>0.05)。结论CHD患者肠道�Objective To investigate the correlation between the amount of Lactobacillus,Bifidobacterium,Escherichia coli in intestinal and the levels of blood lipid,homocysteine(HCY)in patients with coronary heart disease(CHD).Methods A total of 50 patients with CHD admitted to the First Affiliated Hospital of Xinxiang Medical University from May2019 to August 2019 were selected as the observation subjects(CHD group),and 30 healthy physical examination subjects in the same period were selected as the control group.The 5 mL elbow venous blood was obtained from all subjects at the next morning after admission,and the levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL),low-density lipoprotein(LDL)and HCY were detected by automatic biochemical analyzer.Fresh stool samples(5.0 g)were collected from all subjects before treatment.Total bacterial RNA was extracted by using the bacterial RNA extraction kit.The amount of Lactobacillus,Bifidobacterium and Escherichia coli were detected by real-time fluorescence quantitative polymerase chain reaction.The lation between the amount of the intestinal flora and the serum levels of TC,TG,HDL,LDL and HCY was analyzed.Results Compared with the control group,the levels of serum TC,TG,LDL and HCY in the CHD group were significantly increased,and the level of HDL was significantly decreased(P<0.05).Compared with the control group,the amount of Bifidobacteria and Lactobacillus in feces of subjects in the CHD group were significantly reduced,and the amount of Escherichia coli was significantly increased(P<0.05).The levels of serum TC of the subjects in the two groups were negatively correlated with the amount of Bifidobacteria(P<0.05),but there was no correlation between the levels of serum TC and the amount of Lactobacillus and Escherichia coli(P>0.05).The levels of serum TG of the subjects in the two groups were negatively correlated with the amount of Bifidobacteria(P<0.05),and were positively correlated with the amount of Escherichia coli(P<0.05),but not correlated w

关 键 词：血脂 同型半胱氨酸 肠道菌群 冠状动脉性心脏病 

分 类 号：R541.4[医药卫生—心血管疾病]