7个高血压药物相关基因的多态性在582例汉族高血压患者中的分布  

Distribution of seven hypertension drug-related gene polymorphisms in 582 Han patients with hypertension

作　　者：喻晶[1] 杨璐 张舒琼 王秋[1] 姜航 YU Jing;YANG Lu;ZHANG Shuqiong;WANG Qiu;JIANG Hang(Department of Clinical Laboratory,Shenzhen Hospital,Peking University,Shenzhen,Guangdong 518029,China)

机构地区：[1]北京大学深圳医院检验科,广东深圳518029

出　　处：《国际检验医学杂志》2021年第3期325-328,333,共5页International Journal of Laboratory Medicine

基　　金：深圳市三名工程项目(SZSM201812088)。

摘　　要：目的研究7个高血压药物相关基因的多态性在汉族高血压患者中的分布,为高血压患者的个体化治疗提供依据。方法以2017年8月至2019年4月在该院住院的582例汉族高血压患者为研究对象,采用多重荧光定量PCR方法检测WHO推荐的5类治疗高血压药物的7个相关基因的多态性,研究其基因在高血压患者中的频率分布情况,分析其在不同性别和不同地域的分布。结果582例高血压患者CYP2D6*10、ADRB1(1165G>C)、CYP2C9*3、AGTR1(1166A>C)、ACE(I/D)、NPPA(T2238C)、CYP3A5*3基因的突变率分别为55.76%、74.14%、3.69%、4.55%、33.33%、1.03%、72.94%。354例男性和228例女性高血压患者相比,7个高血压药物相关基因的突变率差异均无统计学意义(P>0.05)。582例患者按照地域分为东北、华东、华北、华中、华南、西南和西北7个区,不同地域患者7个高血压药物相关基因多态性的分布频率差异无统计学意义(P>0.05)。结论高血压患者CYP3A5*3、CYP2D6*10、ADRB1(1165G>C)突变频率较高,提示对钙离子拮抗剂和β受体阻断剂比较灵敏;NPPA(T2238C)突变频率较低,提示对利尿剂灵敏度相对较低。不同性别的高血压患者7个高血压药物相关基因多态性分布无明显差异。Objective To study the distribution of polymorphisms of seven hypertension drug-related genes in Han hypertension patients,and to provide a basis for individualized treatment of hypertension patients.Methods In this study,582 Han patients with hypertension from August 2017 to April 2019 in the hospital were selected as the research objects.The polymorphisms of seven genes related to five kinds of antihypertensive drugs recommended by WHO were detected by multiplex fluorescent quantitative PCR.The distribution of gene frequency in patients with hypertension was studied,and the distribution in different genders and regions was analyzed.Results The gene mutation rates of CYP2D6*10,ADRB1(1165G>C),CYP2C9*3,AGTR1(1166A>C),ACE(I/D),NPPA(T2238C),CYP3A5*3 were 55.76%,74.14%,3.69%,4.55%,33.33%,1.03%and 72.94%,respectively.There was no significant difference in mutation rate of seven hypertension drug-related genes between 354 male and 228 female patients with hypertension(P>0.05).Totally 582 patients were divided into seven regions according to their regions:Northeast China,East China,North China,central China,South China,Southwest China and Northwest China,there were no significant differences in the distribution frequency of seven hypertension drug-related genes polymorphisms among different regions(P>0.05).Conclusion The mutation frequencies of CYP3A5*3,CYP2D6*10 and ADRB1(1165G>C)were higher in patients with hypertension,suggesting that they were more sensitive to calcium antagonists and beta blockers;while NPPA(T2238C)mutation frequency was relatively low,suggesting that the sensitivity to diuretics was relatively low.There were no significant differences in seven hypertension drug-related genes polymorphisms distribution between different genders.

关 键 词：高血压 高血压药物相关基因 基因多态性 个体化治疗 

分 类 号：R554[医药卫生—血液循环系统疾病]