不同年龄段银杏树根系土壤微生物群落功能多样性分析  

Functional diversity of soil microbial community in the root soil of Ginkgo biloba tree at different ages

作　　者：陈嘉慧[1] 钱叶[1] 侯怡铃[1] 张萌[1] 丁祥 CHEN Jiahui;QIAN Ye;HOU Yiling;ZHANG Meng;DING Xiang(Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education,College of Life Science,China West Normal University,Nanchong 637009,China;College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,China)

机构地区：[1]西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充637009 [2]西华师范大学环境科学与工程学院,四川南充637009

出　　处：《生物加工过程》2021年第1期85-90,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家级大学生创新创业训练计划(201710638001);2016年校级大学生创新训练项目(cxcy2016018)。

摘　　要：为研究不同年龄段银杏树根系土壤微生物群落代谢功能多样性特征规律,运用Biolog生态板(ECO)技术,结合主成分分析(PCA)和香农韦弗(Shannon⁃Weaver)指数(H)、丰富度指数(S)、均匀度指数(E)、McIntosh指数(U)等研究幼年期(10年以下)、挂果期(20年)和盛果期(40年以上)银杏树根系表层土壤微生物群落功能多样性变化。结果表明:银杏树根系土壤微生物平均颜色变化率(AWCD)增长速率和最大值有显著差异,并呈现幼年期>盛果期>挂果期的趋势;其主要碳源利用类型是氨基酸类、酯类、胺类和酸类碳源;PCA分析表明,3个年龄段银杏树根系土壤微生物碳源利用特征呈现差异性。研究表明,银杏树的年龄段会影响其根系土壤微生物的群落代谢能力和碳源利用能力。In order to characterize the metabolic functional diversity of soil microbial community in the root system of Ginkgo biloba tree at different ages,we use Biolog⁃ECO board technology,combined with PCA(principal component analysis)and Shannon⁃Weaver index(H),richness index(S),evenness index(E),McIntosh index(U),to study the functional diversity changes of surface soil microbial community in root system of Ginkgo biloba tree in the Juvenile period(less than 10 years),Period of hanging fruit(20 years),period of full fruit(40 years or more).The average well color development(AWCD)growth rate and maximum value of soil microorganisms in the root system of Ginkgo biloba tree were significantly different,and showed the trend of juvenile period>Period of full fruit>Period of hanging fruit􀆰The main types of carbon sources are amino acids,esters,amines and acids.The principal component analysis showed that the carbon source utilization characteristics of the soil microbial biomass of Ginkgo biloba tree at different ages were different.The age of Ginkgo biloba tree affected the metabolic ability and carbon resource utilization ability of soil microbial community.

关 键 词：银杏 土壤微生物 Biolog⁃ECO 群落多样性 主成分分析 碳源 

分 类 号：Q938􀆰[生物学—微生物学]