三角梅cDOPA 5GT基因的克隆和光照对其表达的影响  

Cloning of Cyclo-DOPA-5-glucosyltransferase Gene from Bougainvillea glabra and Effect of Illumination on Its Expression

作　　者：刘姗 孙蓉 高静雷 LIU Shan;SUN Rong;GAO Jing-lei(Key Laboratory of Dry-hot Valley Characteristic Bio-Resources Development at University of Sichuan Province,Department of Biological and Chemical Engineering,Panzhihua University,Panzhihua 617000,Sichuan,China)

机构地区：[1]攀枝花学院生物与化学工程学院,干热河谷特色生物资源开发四川省高校重点实验室,四川攀枝花617000

出　　处：《热带亚热带植物学报》2021年第1期75-81,共7页Journal of Tropical and Subtropical Botany

基　　金：四川省国际科技合作项目(2019YFH0136)资助。

摘　　要：为了解光照对三角梅(Bougainvillea glabra)葡糖基转移酶基因表达的影响,从其苞片中克隆了环多巴5-糖基转移酶基因(cDOPA 5GT)。结果表明,三角梅cDOPA 5GT基因的cDNA全长为1446 bp,编码482个氨基酸,生物信息学分析表明cDOPA 5GT蛋白的等电点(PI)为5.77,具有糖基转移酶的特征基序,为酸性亲水跨膜蛋白,不含信号肽,二级结构以α-螺旋和无规则卷曲为主,氨基酸序列保守性较差。qRT-PCR分析表明,遮光处理cDOPA 5GT基因表达量和植株的甜菜红素含量均显著下降。这表明三角梅的色素合成受糖基转移酶基因调控,并与光照呈正相关关系。In order to understand the effect of illumination on the expression of glucosyltransferase gene in Bougainvillea glabra,the cyclo-DOPA-5-glucosyltransferase gene(cDOPA 5GT)was cloned from bracts.The results showed that the full length cDNA of cDOPA 5GT was 1446 bp,encoding 482 amino acids.The bioinformatics analysis showed that isoelectric point of cDOPA 5GT was 5.77,showing an acidic and hydrophilic transmembrane protein without signal peptide.The cDOPA 5GT protein had characteristic motifs of glucosyltransferase,and the secondary structure was dominant ofα-helices and random coils,and the amino acid sequence was less conserved.The results of qRT-PCR and spectrophotometry showed that the expression of cDOPA 5GT gene and betacyanin accumulation in plants decreased significantly under shading by qRT-PCR and spectrophotometry.Therefore,it was suggested that the synthesis of betacyanin was regulated by glucosyltransferase gene,and positively correlated with light time.

关 键 词：三角梅 葡糖基转移酶 基因表达 光照 

分 类 号：S685.99[农业科学—观赏园艺]