COPD患者血清Cav-1及CXCL12水平检测与并发肺动脉高压的相关性研究  

Correlation between Levels of Cav-1 and CXCL12 in Serum and Pulmonary Hypertension in Patients with COPD

作　　者：王苗 张萍[2] WANG Miao;ZHANG Ping(Department of Clinical Laboratory,the First People’s Hospital of Xianyang City,Shaanxi Xianyang 712000,China;Department of Medical Laboratory,the Central Hospital of Shangluo City,Shaanxi Shangluo 726000,China)

机构地区：[1]咸阳市第一人民医院检验科,陕西咸阳712000 [2]商洛市中心医院医学检验科,陕西商洛726000

出　　处：《现代检验医学杂志》2021年第1期29-32,50,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨血清小窝蛋白-1(caveolave-1,Cav-1)及趋化因子C-X-C基元配体12(chemokine C-X-C ligand 12,CXCL12)水平与慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)并发肺动脉高压(pulmonary hypertension,PH)的相关性。方法收集2019年3月~2020年3月期间在咸阳市第一人民医院和商洛市中心医院就诊的115例COPD患者作为研究对象,依据患者是否并发PH分为非PH组(NPH组,70例)和PH组(45例)。另选取43例同期体检健康者作为对照组,采用酶联免疫吸附法测定血清Cav-1和CXCL12水平,所有患者均接受肺功能检测,用肺功能检测仪检测患者治疗前的1s用力呼气容积占预计值的百分比(forced expiratory volume/predicted value,FEV1/Pre),FEV1占用力肺活量(Forced vital capacity,FVC)的百分比(FEV1/FVC)。通过彩色多普勒超声检查获得所有COPD患者的肺动脉收缩压(pulmonary artery systolic pressure,PASP)。收集患者的临床资料,包括二氧化碳分压(PCO2)、氧分压(PO2)、B型脑利钠肽(brain natriuretic peptide,BNP)以及白介素-6(interleukin-6,IL-6)等,比较分析以上指标的变化与COPD并发PH的相关性。结果在对照组,NPH组和PH组中,血清Cav-1和CXCL12水平分别为10.37±2.28,7.23±1.72,4.81±0.90μg/L和65.74±12.27,175.36±26.19,270.33±43.79pg/ml,与对照组比较,NPH组和PH组的CXCL12水平明显增高,且PH组增高更显著;与对照组比较,NPH组和PH组的Cav-1水平则明显降低,且PH组降低更显著,差异均有统计学意义(F=103.71~130.67,均P=0.000)。相关性分析显示,NPH组和PH组的血清Cav-1及CXCL12水平分别呈负相关性(r=-0.813,-0.827,均P<0.01),NPH组的CXCL12水平分别与PCO2,PASP,IL-6及BNP水平呈正相关性,而与FEV1/Pre,FEV1/FVC及PO2呈负相关性。NPH组的Cav-1分别与PCO2,PASP,IL-6及BNP水平呈负相关性,而与FEV1/Pre,FEV1/FVC及PO2呈正相关性(rCXCL12=0.845,0.810,0.807,0.783,-0.799,-0.775和-0.793,均P<0.01;rCav-1=-0.853,-0.828,-0.816,-0.792,0.763,0.803,0.822,均P<0.01)。PH�Objective To investigate the correlation between caveolave-1(Cav-1)and chemokine C-X-C ligand 12(CXCL12)levels in serum and chronic obstructive pulmonary disease(COPD)complicated with pulmonary hypertension(PH).Methods A total of 115 COPD patients from March 2019 to March 2020 in the the first people’s Hospital of Xianyang city and the Central Hospital of Shangluo City were selected as the research objects.According to whether the patients complicated with PH,they were divided into non PH group(NPH group,70 cases)and pH group(70 cases).The serum levels of Cav-1 and CXCL12 were measured by enzyme-linked immunosorbent assay(ELISA).All patients received pulmonary function test.The forced expiratory volume/predicted value(FEV1/pre)and FEV1/FVC of forced vital capacity(FVC)were measured by pulmonary function analyzer,and pulmonary artery systolic pressure(PASP)was obtained by color dppler ultrasound.The clinical data of patients were collected,including partial pressure of carbon dioxide(PCO2),partial pressure of oxygen(PO2),brain natriuretic peptide(BNP)and interleukin-6(IL-6).The correlation between the changes of the above indexes and COPD complicated with pH was analyzed.Results The serum levels of Cav-1 and CXCL12 were 10.37±2.28,7.23±1.72,4.81±0.90μg/L and 65.74±12.27,175.36±26.19,270.33±43.79 pg/ml in the control,NPH and PH groups,respectively.Compared with the control group,the level of CXCL12 in NPH group and PH group was increased,and the increase in PH group was more significant;However,Cav-1 level decreased significantly,and PH group decreased more significantly.Correlation analysis showed that serum levels of Cav-1 and CXCL12 in NPH group and PH group were negatively correlated(r=-0.813,-0.827,P<0.01).CXCL12 level of NPH group was positively correlated with PCO2,PASP,IL-6 and BNP,and was negatively correlated with FEV1/pre,FEV1/FVC and PO2。An opposite trend was presented for Cav-1 level of NPH group(rCXCL12=0.845,0.810,0.807,0.783,-0.799,-0.775 and-0.793,all P<0.01.rCav-1=-0.853,-0.828,-0.816,-

关 键 词：慢性阻塞性肺疾病 肺动脉高压 肺动脉收缩压 肺功能检测 小窝蛋白-1 趋化因子C-X-C基元配体12 白介素-6 B型脑利钠肽 

分 类 号：R563[医药卫生—呼吸系统] R392.11[医药卫生—内科学]