高通量测序技术检测孕早期自然流产绒毛样本染色体异常发生的Meta分析  

Incidence of the Chromosomal Abnormality of the Early Abortion Villi Samples by the Next Generation Sequence:A Meta-Analysis

作　　者：辛淑文 杨少哲 胥博 滕少侠[1] 李荣香[1] 付秀虹[1] XIN Shu-wen;YANG Shao-zhe;XU Bo;TENG Shao-xia;LI Rong-xiang;FU Xiu-hong(Center for Reproductive Medicine and Genetics of Luohe Central Hospital,Henan Luohe 462000,China)

机构地区：[1]漯河市中心医院生殖医学与遗传中心,河南漯河462000

出　　处：《现代检验医学杂志》2021年第1期38-43,共6页Journal of Modern Laboratory Medicine

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20191418);漯河医学高等专科学校2020年度创新创业发展能力提升工程科研类项目(2020-LYZKYYB021)。

摘　　要：目的通过单组率的Meta分析,探索早期流产绒毛样本染色体异常发生情况。方法以自然流产、高通量测序技术等为检索词在CNKI,Pubmed等相关中英文数据库等进行检索,筛选纳入高通量测序技术检测孕早期自然流产绒毛样本染色体异常发生情况的文献,采用Revman 4.3软件进行荟萃分析。结果该研究共纳入12篇文献,723例样本。Meta分析结果显示:在723例孕早期流产绒毛样本中,染色体异常共计477例,总体异常率为68%(0.58~0.76),染色体数目异常率为50%(0.43~0.57),染色体微缺失/微重复异常率为16%(0.09~0.24)。结论高通量测序可以作为检测孕早期绒毛样本染色体异常的重要手段,染色体异常是孕早期自然流产的重要原因。Objective To explore the incidence of the chromosomal abnormality of the early abortion villi samples using the next generation sequence with a Meta-analysis.Methods The CNKI,Pubmed and other related Chinese and English database were retrieved to screen the literature that included the incidence of the chromosomal abnormality of the early abortion villi samples using the high throughput sequencing.Revman 4.3 software was used for meta-analysis.Results A total of 12 articles were included in the analysis,which includes 723 early abortion villi samples.Meta-analysis showed that there were 477 samples of chromosomal abnormality in 723 early abortion villi samples,the overall abnormal rate was 68%(0.58~0.76),the incidence rates of chromosomal abnormal number were 50%(0.43~0.57),and the incidence rates of chromosomal segmental deletion or duplication was 16%(0.09~0.24).Conclusion The next generation sequence technology can be used as the key means to detect the abnormality chromosomal in these early abortion villi samples.The abnormality of chromosomal is an important cause of spontaneous abortion in early pregnancy.

关 键 词：高通量测序 自然流产 染色体 META分析 

分 类 号：R714.21[医药卫生—妇产科学] R446.7[医药卫生—临床医学]