HPLC法测定毛竹笋中核苷类成分的含量  

Determination of Nucleosides in Shoots of Phyllostachys Edulis by HPLC

作　　者：陈笑天[1] 李聪 胡慧明[1] 杜文鹏 邓雅琼[1] 李斌[1] CHEN Xiao-tian;LI Cong;HU Hui-ming;DU Wen-peng;DENG Ya-qiong;LI Bin(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;The Second Affiliated Hospital of Nanchang University,Nanchang 330006)

机构地区：[1]江西中医药大学,南昌330004 [2]南昌大学第二附属医院,南昌330006

出　　处：《江西中医药大学学报》2021年第1期82-84,共3页Journal of Jiangxi University of Chinese Medicine

基　　金：江西省教育厅科技项目(GJ171550);江西省江西省卫计委中医药科研项目(2017B078)。

摘　　要：目的:建立HPLC法测定毛竹笋中尿嘧啶、尿苷、胸腺嘧啶、腺苷的含量。方法:采用HPLC法,色谱柱为Hypersil C18柱(4.6 mm×250 mm,5μm),以甲醇-水为流动相,梯度洗脱,流速0.5 m L/min,检测波长为254 nm,柱温30℃。结果:尿嘧啶、尿苷、胸腺嘧啶、腺苷分别在5.1~102.0μg/m L、5.0~100.0μg/m L、4.9~98.0μg/m L、5.1~102.0μg/m L的范围内具有良好的线性关系(R^2分别为0.9998,0.9999,0.9997,0.9998);平均加样回收率分别为98.13%,97.55%,97.90%,98.61%;RSD分别为1.15%,0.89%,1.48%,1.92%。结论:该法简单快捷,结果准确,可用于测定毛竹笋中核苷类成分的含量。Objective:To establish the method for the determination of uracil,uridine,thymine and adenosine in shoots of Phyllostachys edulis by HPLC.Methods:HPLC method was adopted.The column was Hypersil C18(4.6 mm×250 mm,5μm),methanol and water as the mobile phase with a flow rate of 0.5 m L/min,with gradient elution,the detection wavelength was 254 nm,and the column temperature was 30℃.Results:The linear range of nucleosides were 5.1~102.0μg/m L、5.0~100.0μg/m L、4.9~98.0μg/m L、5.1~102.0μg/m L(R^2 were 0.9998,0.9999,0.9997,0.9998).The average recovery were 98.13%,97.55%,97.90%,98.61%,the RSD were 1.15%,0.89%,1.48%,1.92%.Conclusion:The method is simple,rapid and accurate,it can be used for the determination of nucleosides in shoots of Phyllostachys edulis.

关 键 词：HPLC 毛竹笋 核苷类成分 含量测定 

分 类 号：R284.1[医药卫生—中药学]