益气活血颗粒治疗慢性心力衰竭的网络药理学探究  

Network Pharmacology Study of Yiqihuoxue Granules in the Treatment of Chronic Heart Failure

作　　者：张燕飞 陈苏宁[1] ZHANG Yanfei;CHEN Suning(Shengjing Hospital of China Medical University,Shenyang 110004,Liaoning,China)

机构地区：[1]中国医科大学附属盛京医院,沈阳110004

出　　处：《中西医结合心脑血管病杂志》2021年第3期371-376,共6页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：中国医科大学新增博士、硕士学位授权学科建设基金(No.MB87)。

摘　　要：目的探讨益气活血颗粒治疗慢性心力衰竭的分子机制,从网络药理学角度分析益气活血颗粒治疗慢性心力衰竭的有效活性成分及潜在作用靶点、相关途径。方法从中药系统药理学数据库及分析平台(TCMSP)数据库中收集和鉴定活性成分,预测其作用靶点。利用基因功能富集分析平台(CTD)筛选心力衰竭相关预测靶点,分别利用蛋白质相互作用网络(STRING)数据库和Cytoscape 3.7.1软件建立蛋白质相互作用网络和药物-成分-靶点网络。利用DAVID 6.8数据库分析靶点京都基因与基因组百科全书(KEGG)通路,通过相关通路的图片突出显示作用靶点。结果共筛选出223个有效成分,包括丹参酮ⅡA、山柰酚、槲皮苷、十八烯酸、柚苷配基、谷固醇、糖甙、甘草素C、鳞叶甘草素A、7-邻甲基异亮醇、常春藤皂苷元、7-甲氧基-2-甲基异黄酮、黄芩素、二氢丹参内酯等。有297个预测靶标和慢性心力衰竭有关,包括TNF、MAPK1、IL-6、BCL-2、JUN、RELA、STAT3、MMP、TP53、Caspase等,根据节点自由度(Degree)大小分析多种相关的基因本体(GO)功能和KEGG通路。结论从益气活血颗粒中共筛选出IL-6、TNF、TP53、VEGFA、HMOX1、IFNG、EDN1、NOS2、MMP2、CCL2等关键靶点,可能通过调节肿瘤坏死因子信号通路等治疗慢性心力衰竭疾病。Objective To explore the molecular mechanism of Yiqihuoxue Granulesin the treatment of chronic heart failure and the effective active components,potential targets and related pathways of Yiqihuoxue Granules in the treatment of chronic heart failure were analyzed from the perspective of network pharmacology.Methods The active ingredients were collected and identified from TCMSP database,and the action targets were predicted.The predictive targets related to heart failure were screened by CTD,and the protein interaction network and drug-component-target network were established respectively by STRING database and Cytoscape3.7.1 software.The target KEGG pathway was analyzed by DAVID6.8 database,and the target was highlighted by the pictures of related pathways.Results Two hundred and twenty-three effective components were screened out,including tanshinoneⅡ,kaempferia galanga phenol,quercetin,A valley of oleic acid,naringin ligands,sterol,glucoside,liquorice,C,leaf licorice root element A and 7-bright methyl isopropyl alcohol,ivy saponins yuan,7-methoxy-2-methyl isoflavones,radix scutellariae,dihydrogen salvia miltiorrhiza lactone and so on.There were 297 predicted targets related to chronic heart failure,including TNF,MAPK1,IL-6,Bcl-2,JUN,RELA,STAT3,MMP,TP53 and Caspase.Various GO functions and KEGG pathways were analyzed according to the degree of freedom.Conclusion Key targets including IL-6,TNF,TP53,VEGFA,HMOX1,IFNG,EDN1,NOS2,MMP2 and CCL2 were selected from Yiqihuoxue GranuleS,which might regulate tumor necrosis factor signaling pathway and treat chronic heart failure.

关 键 词：慢性心力衰竭 益气活血颗粒 分子靶标 网络药理学 

分 类 号：R28[医药卫生—中药学]