DPPH、ABTS和FRAP微量法测定山奈酚的抗氧化能力  

Determination of Antioxidant Effects of Kaempferol by Micro-model of DPPH,ABTS and FRAP Assay

作　　者：王荣[1] 罗倩 冯怡 WANG Rong;LUO Qian;FENG Yi(Department of Pharmacy,Xi an Medical University,Shaanxi Xi an 710021,China)

机构地区：[1]西安医学院药学院,陕西西安710021

出　　处：《广州化工》2021年第3期58-59,63,共3页GuangZhou Chemical Industry

基　　金：西安医学院药学省级重点学科建设项目(2016YXXK09,2016YXXK16);西安医学院国家自然科学基金培育项目(2017GJFY13);西安医学院国家级、省级和校级大学生创新创业训练项目(201911840015,121519015)。

摘　　要：建立微量DPPH自由基和ABTS自由基清除法评价山奈酚对自由基清除能力的大小;建立微量总抗氧化能力法评价山奈酚的抗氧化活性强弱。山奈酚对DPPH和ABTS自由基的清除作用随浓度升高而增大,二者的IC 50分别为16.17和6.97μmol/L,其中山奈酚对ABTS自由基的清除能力与V C相当。山奈酚的总抗氧化能力随浓度升高而增加,当浓度为500μmol/L时其总抗氧化能力为1.04±0.019 mmol/L。山奈酚具有一定的体外抗氧化能力,对DPPH和ABTS自由基均有较好的清除能力。A micro-model of DPPH free radical scavenging assay and ABTS free radical scavenging assay were used to evaluate the scavenging ability of Kaempferol against DPPH free radical and ABTS free radical.The antioxidant activity of Kaempferol was evaluated by measuring a micro-model of FRAP.The scavenges DPPH and ABTS free radicals of Kaempferol increased with the increase of the concentration.IC 50 of the scavenge DPPH and ABTS free radicals were 16.17 and 6.97μmol/L,respectively.The scavenging ability of Kaempferol to ABTS was similar to that of V C.The total antioxidant capacity was significantly correlated with the Kaempferol concentration,and the total antioxidant capacity at the 500μmol/L was 1.04±0.019 mmol/L.Kaempferol has certain antioxidant capacity in vitro and scavenges DPPH and ABTS free radicals.

关 键 词：山奈酚 DPPH ABTS FRAP 抗氧化 

分 类 号：R965.1[医药卫生—药理学]