经脾种植VX2瘤株兔肝转移瘤模型的DSA表现  

DSA manifestation of rabbit liver metastases model of VX2 tumor strain implanted in the spleen

作　　者：吴勇超[1] 李智岗[1] 李杰[1] 赵瑜[1] 李顺宗[1] 武中林[1] WU Yong-chao;LI Zhi-gang;LI Jie;ZHAO Yu;LI Shun-zong;WU Zhong-lin(Department of Radiological Intervention, the Fourth Hospital of Hebei Medical Universitiy, Shijiazhuang 050011, China)

机构地区：[1]河北医科大学第四医院放射介入科,河北石家庄050011

出　　处：《河北医科大学学报》2021年第1期85-89,94,共6页Journal of Hebei Medical University

基　　金：国家癌症中心肿瘤科研专项课题(NCC2017A26);河北省科技厅重点研发计划项目(18277744D);河北省医学科学研究重点课题计划(20170740);河北省中医药类科研计划课题(2019135);河北省科技计划项目(162777216)。

摘　　要：目的分析经脾种植VX2瘤株兔肝转移瘤(liver metastases,LM)模型的数字减影血管造影(digital subtraction angiography,DSA)表现,探讨该模型能否成为动脉灌注化疗/栓塞(hepatic artery infusion chemotherapy/transcatheter arterial chemoembolization,HAI/TACE)治疗LM的理想动物模型。方法选取新西兰大白兔40只,建立经脾种植VX2瘤株兔LM模型。采用直视方法经右侧股动脉穿刺,将微导管选入肝动脉内,行肝动脉DSA,观察VX2瘤株兔LM的供血血管及染色情况;行肝动脉锥形束强化CT,观察VX2瘤株兔LM的动脉强化方式,并行HE染色,镜下观察组织学表现。结果成功建立VX2瘤株兔LM模型37只,均行股动脉穿刺插管,1次穿刺插管成功32只,穿刺成功率为86.5%(32/37)。DSA发现LM病灶57个,其中中等血供38个、乏血供19个。DSA表现为肝内动脉走行迂曲,LM的肿瘤供血支紊乱、不规则,动脉中晚期可见多发LM染色,呈类圆形,中心区域染色较边缘染色浅淡,实质期LM染色进一步加重,但是染色程度低于正常肝实质。肝动脉锥形束强化CT表现为多发大小不等LM,强化特点为转移瘤周边呈环形强化,中心区域未见强化。LM的DSA染色部分、肝动脉锥形束CT肿瘤强化部位及程度与腹部增强CT的LM强化表现一致,为LM周边强化,对应于HE镜下观察肿瘤外周存在活性的肿瘤细胞、炎性细胞及结缔组织;LM的DSA中心染色浅淡、肝动脉锥形束CT未强化部位与腹部增强CT的LM未强化表现一致,对应HE镜下肿瘤中心分布嗜碱性红染坏死肿瘤细胞。结论经脾种植VX2瘤株兔LM模型符合临床上影像学表现为环形强化、DSA下为中等血供或乏血供的LM,能提供临床上研究中等血供或乏血供LM的HAI/TACE治疗疗效实验研究的理想动物模型,值得在基础或临床实验中推广和应用。Objective To analyze the manifestations of the digital subtraction angiography(DSA)in rabbit liver metastases(LM)model of VX2 tumor strain implanted in the spleen,and to explore whether the model could be an ideal animal model for the treatment of liver metastases by hepatic artery infusion chemotherapy/transcatheter arterial chemoembolization(HAI/TACE).Methods A total of 40 New Zealand white rabbits were selected to establish the rabbit LM model of VX2 tumor strain implanted in the spleen.The right femoral artery was punctured under direct vision,and the microcatheter was advanced into the hepatic artery.The hepatic artery DSA was performed to observe the blood supply and staining of rabbit LM of the VX2 tumor strain,and then the hepatic artery cone-beam enhanced CT was performed to observe arterial enhancement method of rabbit LM of the VX2 tumor strain,as well as the histological appearance under the HE staining microscope.Results A total of 37 rabbits LM models of VX2 tumor strain were successfully established,all of which underwent femoral artery puncture and cannulation.Of them,32 were successful in one puncture and cannulation,and the success rate of puncture was 86.5%(32/37).DSA revealed 57 LMs in rabbit VX2 tumor strain,including 38 with moderate blood supply and 19 with insufficient blood supply.The DSA manifestations included tortuous intrahepatic artery,and the tumor blood supply branch of LM was disordered and irregular.The multiple LM staining could be seen in the middle and late stages of the artery,which had circular shape.The central area staining was lighter than the edge staining,and the parenchymal LM staining was further aggravated,but the staining was less than normal liver parenchyma.Cone-beam enhanced CT of hepatic artery showed multiple LMs of different sizes.The enhancement was characterized by a ring-shaped enhancement around the metastatic tumor and no enhancement in the central area.The DSA stained part of the LM,the location and degree of tumor enhancement of the hepatic artery cone

关 键 词：肝转移瘤 肿瘤种植 数字血管减影 

分 类 号：R735.7[医药卫生—肿瘤]