乙醇浸提法提取红叶李中总黄酮工艺优化  

Optimization of extraction process of total flavonoids in prunus cerasifera by ethanol extraction

作　　者：程时劲[1] 余渤 毛博 许星星 李安迪 刘晓霞 CHENG Shi-jing;YU Bo;MAO Bo;XU Xing-xing;LI An-di;LIU Xiao-xia(College of Life Science and Chemistry,Wuhan Donghu University,Wuhan 430212,China)

机构地区：[1]武汉东湖学院生命科学与化学学院,武汉430212

出　　处：《湖北农业科学》2021年第2期134-136,共3页Hubei Agricultural Sciences

基　　金：2018年度湖北省教育厅科研计划项目(B2018283);武汉东湖学院2019年度“东湖未来之星”大学生科研项目(重点项目8)。

摘　　要：对红叶李中总黄酮的提取工艺进行了优化,采用乙醇浸提法提取粗提物,用紫外可见分光光度计进行了检测。结果表明,提取溶剂不同、改变提取溶剂的浓度、改变料液比、浸提时的温度和提取时间不同都会对红叶李中总黄酮提取效果产生影响,当乙醇浓度为70%、料液比为1∶45(g∶mL),在70℃的恒温水浴箱中浸提70 min,此时红叶李中的总黄酮提取率最高,可达8.24%。The extraction process of total flavonoids in prunus cerasifera was optimized in this paper.Crude extracts were extracted by ethanol leaching method.The detection was carried out with an ultraviolet visible spectrophotometer.The results showed that different extraction solvent,changing the concentration of extraction solvent,changing the ratio of material to liquid,changing the temperature and extraction time would affect the extraction effect of total flavonoids in prunus cerasifera.When the concentration of ethanol was 70%,the ratio of material to liquid was 1∶45,and the extraction time was 70 min in a constant temperature water bath at 70℃,the extraction rate of total flavonoids in prunus cerasifera was the highest,which can reach 8.24%.

关 键 词：红叶李 总黄酮 提取工艺 乙醇浸提法 

分 类 号：S567.19[农业科学—中草药栽培]