小麦耐热分子标记Xwmc44的鉴定及分析  

Identification and Analysis of Heat-tolerant Molecular Marker Xwmc44 in Wheat

作　　者：刘子会[1] 刘畅[2] 彭义峰[3] 李国良[1] 张华宁[1] 张红梅[1] 段硕楠[1] 郭秀林[1] LIU Zi-hui;LIU Chang;PENG Yi-feng;LI Guo-liang;ZHANG Hua-ning;ZHANG Hong-mei;DUAN Shuo-nan;GUO Xiu-lin(Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051;College of Plant Protection,Nanjing Agricultural University,Nanjing 210095;Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050000)

机构地区：[1]河北省农林科学院遗传生理研究所/河北省植物转基因中心重点实验室,石家庄050051 [2]南京农业大学植物保护学院,南京210095 [3]石家庄市农林科学研究院,石家庄050000

出　　处：《植物遗传资源学报》2021年第1期95-101,共7页Journal of Plant Genetic Resources

基　　金：河北省现代农业创新工程(494-0402-YBN-S2XB,494-0402-YBN-CTGQ);河北省重点研发计划项目(20326309D)。

摘　　要：耐热育种是我国小麦育种的一个重要目标。寻找与目标性状关联的分子标记,可克服常规育种的盲目性,提高分子标记辅助选择育种的准确性。本研究对169份小麦品种(系)的千粒重、籽粒长度、籽粒宽度、籽粒表面积和籽粒周长5个籽粒相关性状的耐热指数进行鉴定,通过简单关联分析获得耐热相关分子标记Xwmc44。聚丙烯酰胺凝胶电泳分析表明:Xwmc44具有Ⅰ、Ⅱ、Ⅲ3个等位变异类型。Ⅰ型等位变异与千粒重、籽粒宽度、籽粒表面积和籽粒周长4个籽粒性状的热感指数显著正相关(P<0.05),Ⅰ型等位变异小麦品种(系)的热感指数平均值大于1;Ⅱ型等位变异与千粒重热感指数极显著负相关(P<0.01),与籽粒宽度和籽粒周长2个籽粒性状的热感指数显著负相关(P<0.05),Ⅱ型小麦品种(系)的耐热指数平均值小于1,为优良等位变异;Ⅲ型等位变异与小麦籽粒性状热感指数都不相关。研究结果表明:Xwmc44与小麦耐热性密切相关,其中Ⅱ型等位变异是耐热优良等位变异。这为小麦耐热分子育种提供了参考信息。High temperature at reproductive stage destabilizes grain yield and quality especially in warm climate wheat production region.Breeding for heat-tolerant wheat varieties is an important target of wheat breeding in China,while identification of the molecular markers associated with the heat tolerant traits can accelerate this process.The heat-tolerant indexes of five kernel traits including thousand kernel weight,kernel length and kernel width,kernel surface area and kernel circumference were investigated by taking use of 169 wheat varieties(lines),and genotyping with SSR marker Xwmc44 identified three allelic variantsⅠ,ⅡandⅢ.A significant positive correlation between variantⅠand heat-tolerant indexes of four kernel traits(thousand kernel weight,kernel width,kernel surface area and kernel circumference)was observed.The average values of heat-tolerant indexes of kernel traits in wheat varieties(lines)withⅠallelic variation was greater than 1.The wheat varieties with variantⅡwas negatively correlated with the heat tolerance index of thousand kernel weight(P<0.01),and negatively correlated with the heat-tolerance index of two kernel traits including kernel width and kernel circumference(P<0.05).The average value of heat-tolerance indexes of kernel traits in wheat varieties(lines)withⅡallelic variation was less than 1.No detectable association was observed in variantⅢwith the heat-tolerance indexes.These results showed that Xwmc44 was related to heat tolerance of wheat,andⅡallelic variation was a superior allele for heat tolerance of wheat.This will provide more information for heat-tolerant wheat breeding with molecular markers.

关 键 词：小麦 等位变异 分子标记 耐热性 

分 类 号：S512.1[农业科学—作物学]