真菌PH1008胞外多糖抗小鼠阴道白假丝酵母菌感染  

The anti-Candida albicans infection of mouse by extracellular polysaccharide from PH1008

作　　者：王英[1] 贝宁 裴华[2] 杨文[1] 王华民[1] WANG Ying;BEI Ning;PEI Hua;YANG Wen;WANG Hua-min(Laboratory of Pathogenic Biology and Immunology,Hainan Medical University,Haikou 571199;Department of Laboratory,the Second Affiliated Hospital,Hainan Medical University,Haikou 570000;the Fourth Department of Hainan Geriatric Hospital,Haikou 571100,China)

机构地区：[1]海南医学院病原生物学与免疫学实验室,海南海口571199 [2]海南医学院第二附属医院检验科,海南海口570000 [3]海南省老年病医院四科,海南海口571100

出　　处：《基础医学与临床》2021年第2期155-158,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(31260225)。

摘　　要：目的评估从红树林土壤中分离的真菌PH1008胞外多糖在小鼠阴道白假丝酵母菌感染模型中的抗感染效应,为抗假丝酵母菌感染开发新的治疗药物提供思路。方法用真菌PH1008胞外多糖干预小鼠阴道白假丝酵母菌感染模型,提取阴道组织进行转录组基因表达水平测定以评估胞外多糖的抗感染效应。结果增强了肝细胞生长因子、补体1受体等基因的表达水平,且这一上调现象与多个信号传导途径密切相关。结论真菌PH1008胞外多糖局部干预小鼠阴道后可诱导机体产生抗白假丝酵母菌感染效应,相关机制有待于进一步研究。Objective To evaluate the anti-infective effect of polysaccharide isolated from fungus PH1008 in vaginal Candida albicans infection model of mouse,and to provide a new and potential pathway to the development of anti-candida drugs.Methods Determination of gene expression level of transcriptome in mice vagina after yeast infection mouse model was treated by exopolysaccharide of fungus PH1008.Results The expression of hepatocyte growth factor and complement 1 receptor was increased,and the up-regulation was related to varied signal transduction pathways.Conclusions The anti-infection effect of Candida albicans can be induced by vaginal mucosal immunization with exopolysaccharide of fungus PH1008,and the relevant mechanism needs further research.

关 键 词：红树林真菌 感染 阴道 

分 类 号：Q939.91[生物学—微生物学] R379.9[医药卫生—病原生物学]