聚醚醚酮/58S生物活性玻璃骨植入材料的成骨活性  

Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant

作　　者：刘吕花[1] 郑延延 张丽芳[2] 熊成东[2] LIU Lyu-hua;ZHENG Yan-yan;ZHANG Li-fang;XIONG Cheng-dong(School of Basic Medical Sciences,North Sichuan Medical College,Nanchong 637000;Chengdu Institute of Organic Chemistry,Chinese Academy of Sciences,Chengdu 610041,China)

机构地区：[1]川北医学院基础医学院,四川南充637000 [2]中国科学院成都有机化学研究所,四川成都610041

出　　处：《基础医学与临床》2021年第2期209-213,共5页Basic and Clinical Medicine

基　　金：南充市市校科技战略合作专项资金项目(18SXHZ0114,19SXHZ0246)。

摘　　要：目的探讨聚醚醚酮/58S生物活性玻璃(PEEK/58S)骨植入材料的成骨活性。方法MG-63成骨细胞在PEEK和PEEK/58S表面培养一段时间后,扫描电镜(SEM)观察MG-63成骨细胞的黏附铺展状态;CCK-8法检测MG-63成骨细胞的增殖活性;碱性磷酸酶(ALP)活性检测评价MG-63成骨细胞的成骨分化能力;实时荧光定量PCR(RT-qPCR)检测成骨分化相关基因ALP、BMP-2、COL-I、OCN、OPN mRNA表达。PEEK和PEEK/58S植入大鼠胫骨近端12周后,组织学染色和生物力学拔出实验评价骨植入材料与周围骨组织之间的骨整合能力。结果与纯PEEK相比,PEEK/58S更有利于MG-63成骨细胞的黏附铺展,能显著改善MG-63成骨细胞的增殖活性和ALP活性(P<0.05),显著促进ALP、BMP-2、COL-I、OCN、OPN mRNA的表达(P<0.05)。PEEK/58S比纯PEEK表现出更好的骨整合能力。结论PEEK/58S骨植入材料能显著改善成骨细胞的黏附、铺展、增殖与成骨分化活性,增强其与周围骨组织之间的骨整合能力,有望作为新型骨植入材料用于临床。Objective To explore the osteogenic activity of polyetheretherketone/58S bioactive glasses(PEEK/58S)as a kind of bone implant.Methods MG-63 osteoblasts were cultured on PEEK and PEEK/58S surfaces for predetermined time.Then MG-63 osteoblast attachment and spreading condition were observed by scanning electron microscopy(SEM).The proliferation activity of MG-63 osteoblast was evaluated by CCK-8(cell counting kit).Alkaline phosphatase(ALP)activity was measured to determine the osteogenic differentiation of MG-63 osteoblast.The mRNA expression levels of osteogenesis-related genes ALP,BMP-2,COL-I,OCN and OPN were detected using real-time fluorescent quantitative PCR.Osseointegration of PEEK and PEEK/58S bone implant was evaluated by histological analysis and biomechanical pull-out tests after implantation in a rat tibia model for 12 weeks.Results Compared with the PEEK,the adhesion and spreading of MG-63 osteoblast was more favorable.The proliferation activity and ALP activity of MG-63 osteoblast was significantly promoted(P<0.05).Also,the mRNA expression of ALP,BMP-2,COL-I,OCN and OPN was significantly up-regulated(P<0.05).In vivo experiments showed that PEEK/58S exhibited better osseointegration than pure PEEK.Conclusions PEEK/58S bone implant significantly improves the attachment,spreading,proliferation and differentiation of osteoblast and enhances the peri-implant osseointegration,so it is a potential novel orthopedic material for clinical use.

关 键 词：聚醚醚酮 58S生物活性玻璃 生物活性 成骨活性 骨整合 

分 类 号：R318.08[医药卫生—生物医学工程]