张家口地区机采血小板捐献者HPA分型研究  

Platelet HP A Typing of Platelet Donors in Zhangjiakou Area

作　　者：姜文静[1] 徐云鹏 李菲 李萍[1] 康少平[1] 李立宏[1] 王奕浩 JIANG Wen-Jing;XU Yun-Peng;LI Fei;LI Ping;KANG Shao-Ping;LI Li-Hong;WANG Yi-Hao(College of Laboratory Medicine in Hebei North University;Blood Center of Zhangjiakou Area in Hebei Province;Department of Blood Transfusion,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei Province,China)

机构地区：[1]河北北方学院医学检验学院 [2]河北省张家口市中心血站 [3]河北北方学院第一附属医院输血科,河北张家口075000

出　　处：《中国实验血液学杂志》2021年第1期233-238,共6页Journal of Experimental Hematology

基　　金：河北省教育厅青年基金(QN2016271)。

摘　　要：目的:分析河北省张家口地区血小板捐献者血小板特异性抗原HPA1-5,15系统的基因多态性。方法:采集血小板捐献者静脉血并提取DNA,采用聚合酶链-序列特异性引物(PCR-SSP)法进行血小板特异性抗原分型,计算基因频率、基因型频率并与国内外其他地区进行比较是否存在差异及是否表现地区特异性。结果:HPA-1、HPA-2、HPA-4系统中基因表达均为纯合子aa,未出现表达纯合子bb的存在者,其中HPA-1和HPA-4系统各发现杂合子ab表达1例(1%),HPA-2系统发现杂合子ab表达14例(14%);HPA-5系统基因表达主要为纯合子aa(98%),极少数表达纯合子bb(2%);HPA-3和HPA-15系统基因表达杂合程度较高,HPA-3系统基因表达aa、ab、bb表达比例分别为46%、40%、14%,HPA-15系统基因表达aa、ab、bb表达比例分别为21%、64%、15%。结论:张家口地区血小板特异性抗原HPA1-5,15系统基因频率具有本地区特点;HPA-3和HPA-15系统基因表达杂合程度较高,引发同种免疫及血小板输注无效的可能性较大,因此应引起注意。Objective:To analyze the polymorphism of the HPA1-5,15 system of the donors in Zhangjiakou area.Methods:DNA was extracted from the blood samples of the donors,PCR-SSP method was used to divide HPA1-6,15 genotype.The gene frequency and genotype frequency were calculated,compared with the difference and regiahal specificity of the populations in our country and foregiens was compared other populations.Results:The gene expression in the HPA-1,HPA-2 and HPA-4 systems were all homozygous aa,and the donors who expressed homozygous bb was not exessed.Among them,one heterozygous ab expression was found in both HPA-1 and HPA-4 systems(1%),and 14 cases of heterozygous ab expression were found in HPA-2 system(14%).The gene expression in the HPA-5 system was mainly homozygous aa(98%),and a very few expressed homozygous bb(2%)was found.The degree of heterozygosity of gene expression in the HPA-3 and HPA-15 systems was relatively high.The proprotion of the expression of aa,ab and bb in the HPA-3 system was respectively 46%,40%and 14%,the proprotion of the expression of aa,ab and bb in the HPA-15 system was respectively 21%,64%and 15%.Conclusion:The gene frequency of platelet-specific antigen HPA1-5,15 system in zhangjiakou region shows local characteristics.The heterozygosity degree of gene expression in the HPA-3 and HPA-15 systems are both high,suggesting that they are more likely to result in alloimmunization and ineffective platelet transfusion,which should be pays attention to.

关 键 词：张家口地区 人类血小板特异性抗原 基因型 基因频率 

分 类 号：R457.11[医药卫生—治疗学]