水杨酸对镉胁迫下不结球白菜幼苗生长及生理的响应  

Effects of Salicylic Acid on Growth and Physiology of Non-heading Chinese Cabbage Seedlings under Cadmium Stress

作　　者：陈华[1] 陈永快[2] 王涛 黄语燕 廖水兰 兰婕 康育鑫 CHEN Hua;CHEN Yongkuai;WANG Tao;HUANG Yuyan;LIAO Shuilan;LAN Jie;KANG Yuxin(Fujian Academy of Agricultural Sciences,Fuzhou 350003,Fujian China;Institute of Digital Agriculture,Fujian Academy of Agricultural Sciences,Fuzhou 350003,Fujian China)

机构地区：[1]福建省农业科学院,福建福州350003 [2]福建省农业科学院数字农业研究所,福建福州350003

出　　处：《福建农业学报》2020年第12期1321-1329,共9页Fujian Journal of Agricultural Sciences

基　　金：福建省农业科学院设施专项(A20170-3);福建省农业科学院智慧农业创新团队项目(STIT2017-2-12);福建省农业科学院青年人才自由探索创新项目(AA2018-26);福建省农业科学院科技示范基地项目(sfjd1829);福建省发改委项目(闽发改投资[2018]206号)。

摘　　要：【目的】探讨水杨酸(Salicylic acid,SA)诱导不结球白菜抗镉(Cadmium,Cd)胁迫的响应及对Cd吸收积累和转运差异,为利用SA减轻Cd胁迫对不结球白菜的伤害提供理论依据。【方法】以华冠不结球白菜为试验材料,研究Cd胁迫(50 mg·L^(-1) CdCl·2.5H_2O)及喷施SA(0.01~0.5 mmol·L^(-1))对幼苗生长、光合色素含量、根系活力、渗透调节物质、丙二醛(MDA)含量、抗氧化酶活性和Cd积累的影响。【结果】与空白对照(CK_1)相比,Cd胁迫对照组(50 mg·L^(-1) CdCl·2.5H_2O,CK_2)显著抑制不结球白菜幼苗的生长,降低光合色素含量、根系活力、蛋白质含量、脯氨酸(Pro)含量和抗氧化酶活性,提高丙二醛(MDA)含量。在Cd胁迫下,喷施0.01~0.05 mmol·L^(-1)SA可以促进不结球白菜幼苗生长,以0.05 mmol·L^(-1)SA处理效果最好;在Cd胁迫10 d时,不结球白菜幼苗的根冠比、脯氨酸含量、POD、CAT活性较CK_2提高140%、44.98%、118.18%、70.43%,且显著降低幼苗地上部分Cd含量和转运系数;而喷施0.1~0.5 mmol·L^(-1)SA幼苗生长受抑。【结论】0.05 mmol·L^(-1)SA是最适宜的喷施浓度,能有效缓解Cd对不结球白菜幼苗的毒害,提高幼苗的耐Cd性。【Objective】Growth and physiological responses of non-heading Chinese cabbage to salicylic acid(SA)spraying under Cd-stress were studied.【Method】Huaguan non-heading Chinese cabbage(Brassica chinensis L.)seedlings under an imposed stress of 50 mg CdCl·2.5H2O·L−1 were sprayed with SA at a rate of 0.01-0.5 mmol·L−1.Growth,root activity,osmotic regulators,photosynthetic pigment content,malondialdehyde(MDA)content,and antioxidant enzyme activity as well as the Cd absorption,accumulation,and transportation of the separately treated seedlings were determined.【Result】The seedlings under the Cd-stress control(CK2)grew significantly slower with reduced root vitality,contents of photosynthetic pigments,protein,and proline(Pro),and antioxidant enzyme activities but increased MDA in comparison to the blank control(CK1).Under the Cd-stress,SA spraying in the range of 0.01-0.05 mmol·L−1 rejuvenated the seedling growth,with the greatest effect observed when 0.05 mmol·L−1 SA was applied.The seedlings of CK1 had their root/shoot ratio increased by 140%,proline content by 44.98%,POD by 118.18%,and CAT by 70.43%over those of CK210d under the Cd-stress,while the aboveground parts of the plants had the Cd content and transport coefficient significantly reduced.On the other hand,when higher SA concentrations in the range of 0.1-0.5 mmol·L−1 was applied,the seedling growth was significantly inhibited.【Conclusion】At a concentration of 0.05 mmol SA·L−1,the spraying could effectively alleviate the Cd toxicity and improve the Cd tolerance of the non-heading Chinese cabbage seedlings.

关 键 词：不结球白菜 水杨酸 CD胁迫 抗氧化酶活性 Cd积累 

分 类 号：S634.3[农业科学—蔬菜学]