柳膜肩网蝽和悬铃方翅目网蝽体内微生物多样性研究(半翅目:网蝽科)  

Microbial diversity analysis of Hegesidemus habrus and Corythucha ciliate(Hemiptera:Tingidae)

作　　者：李荣荣 李敏[1] 刘丽洁 徐喆 张虎芳[3] Li Rongrong;Li Min;Liu Lijie;Xu Zhe;Zhang Hufang(Taiyuan Normal University,Jinzhong 030619,China;College of Life Science,Shanxi Normal University,Xi’an 710119,China;Xinzhou Normal University,Xinzhou 034000,China)

机构地区：[1]太原师范学院,山西晋中030619 [2]陕西师范大学生命科学学院,陕西西安710119 [3]忻州师范学院,山西忻州034000

出　　处：《山西农业大学学报（自然科学版）》2021年第1期67-74,共8页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(3150840);山西省高等学校科技创新项目(2020L0511,2019L0803);生态环境部生物多样性调查评估项目(No.2019HJ2096001006);太原师范学院博士启动金。

摘　　要：[目的]探究行道树常见害虫柳膜肩网蝽(Hegesidemus habrus Drake)和悬铃木方翅网蝽(Corythucha ciliate Say)体内微生物群落结构和多样性。[方法]利用Illumina MiSeq(PE300)高通量测序技术对柳膜肩网蝽和悬铃木方翅网蝽体内微生物16S rRNA基因的V3⁃V4特异区域序列进行测序,统计可操作分类单元(operational taxonomic unit,OTU),分析Alpha多样性、物种组成及丰度。[结果]2种网蝽的9个样本共得到366037个有效OTU。Αlpha多样性分析显示柳膜肩网蝽样本的微生物群落丰富度远远高于悬铃木方翅网蝽样本。16S rRNA基因高通量测序显示变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、栖热菌门(Thermi)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和蓝藻门(Cyanobacteria)同时存在于柳膜肩网蝽和悬铃木方翅网蝽样本中。PCA分析可以清楚的将两者样本区分开来。物种及丰度分析显示柳膜肩网蝽的核心菌属包括厌氧芽孢杆菌属(Anoxybacillus)、苍白杆菌属(Ochrobactrum)、栖热菌属(Thermus)、沃尔巴克氏体(Wolbachia);而厌氧芽孢杆菌属、“Candidatus Cardinium”、乳球菌属(Lactococ⁃cus)、苍白杆菌属、沙雷氏菌属(Serratia)及栖热菌属在悬铃木方翅网蝽样本中含量较高。[结论]柳膜肩网蝽和悬铃木方翅网蝽体内微生物组成具有显著差异,但它们都拥有较高丰度的核心菌属——栖热菌属,推测栖热菌属可能是网蝽科昆虫体内普遍存在的共生菌。悬铃木方翅网蝽不同地理种群间菌群组成稳定。沃尔巴克氏体和Cardinium以较高丰度分别存在于柳膜肩网蝽和悬铃木方翅网蝽样本中,为二者的生物防治提供新思路。[Objective]This study was aimed to explore the structure and diversity of the microbial communities in two common pests of landscape trees,Hegesidemus habrus and Corythucha ciliate.[Methods]The V3-V4 specific region genes of the mi⁃crobial 16S rRNA were sequenced by Illumina MiSeq(PE300)high-throughput sequencing technology.The number of OTUs(Operational taxonomic units),the species composition,abundance and alpha diversity of their microbes were then analyzed.[Results]A total of 366,037 valid OTUs were obtained from 9 samples of the two types of species.Alpha diversity analysis suggests that the bacterial community richness of H.habrus was much higher than that of C.ciliate.Next-generation sequenc⁃ing of the 16S rRNA gene revealed the presence of 6 bacterial phyla(Proteobacteria,Firmicute,Thermi,Bacteroidetes,Acti⁃nobacteria,and Cyanobacteria)in both H.habrus and C.ciliate.PCA analysis showed that the bacterial composition varied among interspecific lace bug samples.At genus level,nine core bacteria were found,and H.habrus hosted four of them,in⁃cluding Anoxybacillus,Ochrobactrum,Thermus,and Wolbachia,and C.ciliate had six including Anoxybacillus,"Candidatus Cardinium",Lactococcus,Ochrobactrum,Thermus,and Serratia.[Conclusion]Significant differences of microbial communi⁃ties were observed between H.habrus and C.ciliate.However,we also found that they shared a common core genus,Ther⁃mus,with high abundance.This may indicate that Thermus is the core bacterial that harbor in Tingidae.Wolbachia and Car⁃dinium were found in the samples of H.habrus and C.ciliate,respectively,which may provide new ideas for biological con⁃trol of these two pest species.

关 键 词：柳膜肩网蝽 悬铃木方翅网蝽 16S rRNA高通量测序 菌群组成 

分 类 号：Q965[生物学—昆虫学]