OCT4 通过重编程转录组调节毛囊干细胞分化潜能  

OCT4 REGULATES THE DIFFERENTIATION POTENTIAL OF HAIR FOLLICLE STEM CELLS BY REPROGRAMMING TRANSCRIPTOME

作　　者：余小珍 黄维清[2] 孙鹏鹏 阮迎春 刘志晶 陈桦 YU Xiaozhen;HUANG Weiqing;SUN Pengpeng;RUAN Yingchun;LIU Zhijing;CHEN Hua(Department of Pathology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院病理学系,山东青岛266071 [2]青岛市市立医院病理科 [3]青岛市中心医院重症医学科

出　　处：《青岛大学学报（医学版）》2021年第1期77-81,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金项目(31671076);青岛市科技局资助项目(18-2-2-66-jch)。

摘　　要：目的分析过表达OCT4后人毛囊干细胞中转录本的变化及OCT4靶基因的表达。方法分别收集OCT4转导前后的毛囊干细胞并提取总RNA,利用转录组测序分析全基因转录本的差异,并分析差异表达基因(DEGs)的功能以及OCT4靶基因的表达。结果转导OCT4后的贴壁细胞和悬浮细胞亚群中分别有4283和6206个DEGs,转录本发生显著改变。上调DEGs显著富集于中胚层和外胚层发育的生物过程中,以及调节干细胞多潜能性的信号通路中。多潜能性相关的OCT4靶基因表达显著上调,胚胎造血基因KDR表达水平持续上调。结论OCT4转导后人毛囊干细胞的转录组发生显著改变并获得更多分化潜能,且悬浮细胞亚群呈现造血分化倾向。Objective To investigate the change in transcripts and the expression of the target genes of OCT4 in human hair follicle stem cells after OCT4 overexpression.Methods Total RNA was extracted from hair follicle stem cells before and after OCT4 transduction,and transcriptome sequencing was used to analyze the difference in transcripts.The function of differentially expressed genes(DEGs)and the expression of the target genes of OCT4 were analyzed.Results A total of 4283 and 6206 DEGs were screened out in the adherent cells and suspension cells,respectively,after OCT4 transduction,and there was a significant change in transcripts.Upregulated DEGs were mainly enriched in the biological processes of mesoderm and ectoderm development and the signaling pathways involved in regulating pluripotency of stem cells.The target genes of OCT4 associated with pluripotency were significantly upregulated,and the expression of the embryonic hematopoietic gene KDR continued to increase.Conclusion After OCT4 transduction,human hair follicle stem cells show a significant change in transcriptome and obtain a higher level of pluripotency,and the suspension cells show a tendency of hematopoietic differentiation.

关 键 词：OCT4 毛囊 多潜能干细胞 细胞重新编程 造血干细胞 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]