3种丹参注射剂对大鼠急性心肌缺血的改善作用  

Improvement of Salvia miltiorrhiza injection on acute myocardial ischemia in rats

作　　者：张海啸 于长安[2] 史载祥[1] 杜金行[2] ZHANG Haixiao;YU Chang'an;SHI Zaixiang(Department of TCM,Chuiyangliu Hospital Affiliated to Tsinghua University,Beijing 100022)

机构地区：[1]清华大学附属垂杨柳医院中医科,北京100022 [2]中日友好医院中西医结合心内科,北京100029

出　　处：《河北中医》2020年第11期1685-1689,1699,共6页Hebei Journal of Traditional Chinese Medicine

摘　　要：目的观察3种丹参注射剂对大鼠急性心肌缺血的改善作用。方法将90只SD大鼠按照随机数字表法分为6组,正常组、模型组、注射用丹参(冻干)组、丹参注射液、注射用丹参多酚酸盐组、硝酸甘油注射液组,每组15只。注射用丹参(冻干)组大鼠予注射用丹参(冻干) 4 mg/m L腹腔注射;丹参注射液组大鼠予丹参注射液4 mg/m L腹腔注射;注射用丹参多酚酸盐组大鼠予注射用丹参多酚酸盐1.6 mg/m L腹腔注射;硝酸甘油注射液组大鼠予硝酸甘油注射液0.5 mg/m L腹腔注射。实验药物剂量为成人常用剂量按照人鼠体表面积换算公式换算成等效剂量,各药物临用前用0.9%氯化钠注射液配制成所需浓度,给药容积为10 m L/kg。正常组、模型组大鼠予等容积0.9%氯化钠注射液腹腔注射。均每日1次,连续给药3 d。第3 d给药后1 h,各组大鼠均麻醉后测标准肢体Ⅱ导联心电图作为急性心肌缺血前值。然后注射用丹参(冻干)组、丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠制备急性心肌缺血模型,监测标准肢体Ⅱ导联心电图15 min,采集急性心肌缺血后1、5、10、15 min的心电图为急性心肌缺血后值。观察各组大鼠急性心肌缺血后1、5、10、15 min心电图J点位移变化、T波高度变化;比较各组大鼠血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)变化。结果模型组大鼠急性心肌缺血后1、5、10、15 min心电图J点位移变化、T波高度变化均高于正常组(P<0.05),说明急性心肌缺血模型成功;注射用丹参(冻干)组、丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠急性心肌缺血后5、10、15 min心电图J点位移变化、T波高度变化均低于模型组(P<0.05);注射用丹参(冻干)组大鼠急性心肌缺血后10、15 min心电图J点位移变化、T波高度变化均低于丹参注射液组、注射用丹参多酚酸盐组、硝酸�Objective To observe the improvement of Salvia miltiorrhiza injection on acute myocardial ischemia in rats. Methods 90 SD rats were randomly divided into 6 groups: normal group, model group, Salvia miltiorrhiza lyophilized powder group, Salvia miltiorrhiza parenteral solution group,Salvianolate injection group and nitroglycerin injection group,with 15 rats in each group.Rats in Salvia miltiorrhiza lyophilized powder group were intraperitoneally injected with 4 mg/m L Salvia miltiorrhiza lyophilized powder injection;rats in Salvia miltiorrhiza parenteral solution group were intraperitoneally injected with 4 mg/m L Salvia miltiorrhiza injection;rats in Salvianolate injection group were intraperitoneally injected with 1. 6 mg/m L salvianolate injection;rats in nitroglycerin injection group were intraperitoneally injected with 0. 5 mg/m L nitroglycerin injection. The dosage of the experimental drug was converted into equivalent dose according to dosage commonly used in adults on the basis of the conversion formula of human and mouse body surface area. Before clinical use,the required concentration of each drug was prepared with 0. 9% sodium chloride injection,and the administration volume was 10 m L/kg. The rats in normal group and model group were injected intraperitoneally with equal volume of 0. 9% sodium chloride injection. They were given injection once a day for 3 days. One hour after administration on the third day,the standard II lead ECG was measured as the pre-value of acute myocardial ischemia. Then the acute myocardial ischemia model was established in the four drug administration groups. The standard II lead ECG was monitored for 15 min,and the ECG at 1,5,10 and 15 min after acute myocardial ischemia was collected as the value after acute myocardial is chemia. The changes of J-point displacement and T-wave height of ECG at 1,5,10 and 15 min after acute myocardial ischemia were observed,and the changes of serum creatine kinase( CK),creatine kinase is oenzyme( CK-MB) and lactate dehydrogenase( LDH) were co

关 键 词：心肌缺血 丹参 注射剂 动物实验 

分 类 号：R285.5[医药卫生—中药学] R-331[医药卫生—中医学]