Effect of New Fly Maggot Protein Feed on mRNA Expression of TOR Signaling Pathway-related Genes in Loaches (Misgurnus anguillicaudatus)  

作　　者：Haitao ZHANG Yunlong LI Yongliang CHEN Kailang GU Feng GAO 

机构地区：[1]Xuzhou Vocational College of Bioengineering,Xuzhou 221006,China

出　　处：《Agricultural Biotechnology》2021年第1期9-11,共3页农业生物技术（英文版）

基　　金：Supported by General Project of Natural Science Research in Higher Education Institutions in Jiangsu Province(18KJD240001);Xuzhou Science and Technology Planning Project(KC19200);2018“Qinglan Project”of Jiangsu Higher Education Institutions。

摘　　要：[Objectives] This study was conducted to explore the effects of new fly maggot protein feed on the mRNA expression of genes related to the TOR signaling pathway in loaches(Misgurnus anguillicaudatus). [Methods] Two kinds of test feed with equal nitrogen and energy were prepared by replacing 60% of the fish meal in the control group with the new fly maggot protein feed, i.e., Diet1(control group) and Diet2(60% fish meal replacement group). The feeding experiment was carried out in an indoor circulating water system, and the breeding period was 60 d. [Results] For the livers, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly higher than those in the Diet1 group(P<0.05), while the expression of 4 EBP2 was lower than in the Diet1 group(P<0.05);and as to the muscles, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly lower than those in the Diet1 group(P<0.05), while there was no significant change in the mRNA level of 4EBP2 between the two groups. [Conclusions] The replacement of fish meal by fly maggot cultures affected the mRNA expression of TOR, 4EBP1 and 4EBP2 in loach livers and muscles.

关 键 词：New fly maggot protein feed LOACH TOR signaling pathway 

分 类 号：S966.4[农业科学—水产养殖]