基于血清靶向代谢组学研究蒲地蓝消炎口服液对脂多糖诱导的急性肺炎小鼠能量代谢的影响  

Effect of Pudilan Xiaoyan Oral Liquid on Energy Metabolism in Mice with Acute Pneumonia Induced by LPS Based on Serum Targeted Metabolomics

作　　者：田刚 李超 翟园园 冯丽 徐佳[2] 包贝华[2] 姚卫峰[2] 张丽[2] 丁安伟[2] TIAN Gang;LI Chao;ZHAI Yuan-yuan;FENG Li;XU Jia;BAO Bei-hua;YAO Wei-feng;ZHANG Li;DING An-wei(Jumpcan Pharmaceutical Co.,Ltd.,Taizhou,225411,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,210023,China)

机构地区：[1]济川药业集团有限公司,江苏泰州225411 [2]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2021年第1期120-125,共6页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81973445);江苏省“六大人才高峰”高层次人才项目(2016-YY026);江苏高校“青蓝工程”中青年学术带头人项目(2016)。

摘　　要：目的研究蒲地蓝消炎口服液对脂多糖(LPS)诱导的急性肺炎小鼠能量代谢的影响。方法取ICR小鼠随机分为对照组,模型组,蒲地蓝消炎口服液低、中、高剂量组和阳性药组,灌胃给药,连续给药5 d。GC-MS靶向检测小鼠血清中糖酵解和三羧酸(TCA)循环的12个代谢物(丙酮酸、乳酸、琥珀酸、富马酸、苹果酸、α-酮戊二酸、磷酸烯醇式丙酮酸、顺式乌头酸、3-磷酸甘油酸、柠檬酸、异柠檬酸、葡萄糖),并进行多变量数据分析。结果和对照组比较,模型组小鼠血清中鉴定出乳酸、琥珀酸、苹果酸、α-酮戊二酸、异柠檬酸和葡萄糖等多个显著性差异代谢物,并在蒲地蓝消炎口服液干预下得以有效改善。结论研究精准发现与炎症反应相关的6个显著差异代谢物,为从能量代谢层面上深入阐明蒲地蓝消炎口服液抗呼吸系统炎症的作用机制提供了参考。OBJECTIVE To study the effect of Pudilan Xiaoyan Oral Liquid on energy metabolism in mice with lipopolysaccharide(LPS)-induced acute pneumonia.METHODS ICR mice were randomly divided into control group,model group,Pudilan group and positive drug group,and the drugs were given orally for 5 days.GC-MS was used to detect 12 metabolites(pyruvic acid,lactic acid,succinic acid,fumaric acid,malic acid,α-ketoglutarate,phosphoenolpyruvic acid,cis-aconitic acid,3-phosphoglyceric acid,citric acid,isocitric acid,glucose)in glycolysis and tricarboxylic acid(TCA)cycle in mouse serum and then multivariate data analysis was carried out to search the significant metabolites.RESULTS Among the targeted metabolites,lactic acid,succinic acid,malic acid,α-ketoglutarate,isocitrate and glucose were found as the significant metabolites.Compared to the model group,these metabolites were effectively restored by Pudilan Xiaoyan Oral Liquid.CONCLUSION This study accurately finds six differential metabolites related to inflammation,providing a reference for in-depth explanation of the mechanism of anti-respiratory inflammation of Pudilan Xiaoyan Oral Liquid at the level of energy metabolism.

关 键 词：蒲地蓝消炎口服液 急性肺炎 靶向代谢组学 GC-MS 能量代谢 

分 类 号：R917[医药卫生—药物分析学] R283[医药卫生—药学]