标本存放时间及温度对刺激法Th1/Th2胞内细胞因子检测的影响  

Effects of time and temperature of specimen storage on the results of cytokine Th1/Th2 in peripheral blood

作　　者：苗竹林[1] 钟兴明[1] 崔蓉[1] 李佩佩 王永霞[1] 钟文瑶 赵文忠[1] MIAO Zhu-lin;ZHONG Xing-ming;CUI Rong;LI Pei-pei;WANG Yong-xia;ZHONG Wen-yao;ZHAO Wen-hong(Family Planning Hospital of CGuangulong Prorince,Family Planning Research Institute of lGuangdong Prorince,KeyLaboratory of the National Health and Family Planning Commission for Male Reproduction and Genetics,Guangzhou510600,Guangdong,China)

机构地区：[1]广东省计划生育专科医院、广东省计划生育科学技术研究所国家卫生和计划生育委员会男性生殖与遗传重点实验室,广东广州510600

出　　处：《广东医学》2020年第24期2522-2525,共4页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(2016A030313760)。

摘　　要：目的探讨标本不同存放时间及温度对刺激法检测外周血Th1/Th2胞内细胞因子检验结果的影响。方法对20例乙二胺四乙酸(EDTA)抗凝外周血全血标本放置4℃冰箱及室温(20±5)℃保存,在不同的时间点取出标本,经过PMA+Ion刺激后采用流式细胞诊断技术检测Th1(CD4+/IFN-γ+)和Th2细胞(CD4+/IL-4+),计算Th1/Th2值,对不同温度和不同存放时间的检测结果进行比较。结果标本室温或4℃冰箱放置时,在72 h内检测结果相对稳定,检测结果变化差异无统计学意义(P>0.05)。标本存放3 d后,无论是室温放置,还是在冰箱存放都与抽血当天检测结果比较存在较大的差异(P<0.05)。结论放置时间是影响检验结果的主要因素,而存放温度无明显影响。Objective To investigate the effects of different storage time and temperature on the results of Th1/Th2 test in peripheral blood. Methods Twenty cases of ethylenediaminetetraacetic acid(EDTA) anticoagulant peripheral blood samples were stored in refrigerator at 4℃ and room temperature(20±5)℃. The samples were taken out at different time. After stimulated by PMA + Ion, Th1(CD4^+/IFN-γ^+) and Th2 cells(CD4^+/IL-4^+), the samples were detected by flow cytometry;and Th1/Th2 values were calculated and the detection results at different temperature and storage time were compared. Results When the samples were placed at room temperature or 4℃ refrigerator, the results were relatively stable within 72 hours, and there was no significant difference in the results(P>0.05). After 3 days, there were significant differences between the test results of samples stored at room temperature or in refrigerator and the first day of blood sampling. Conclusion The storage time is the main influence factor on the test results, while the storage temperature has no obvious effect.

关 键 词：流式细胞检测 TH1/TH2 时间 温度 细胞因子 

分 类 号：R711.3[医药卫生—妇产科学] R392.8[医药卫生—临床医学]