色素万寿菊雄性不育植株的快速繁殖  

Rapid Propagation for Male Sterile Lines of Pigment Marigold

作　　者：魏晓梅 吴丽芳 WEI Xiaomei;WU Lifang(College of Biological Resource and Food Engineering,Qujing Normal University/Center for Yunnan Plateau Biological Resources Protection and Utilization,Qujing,Yunnan 655011)

机构地区：[1]曲靖师范学院生物资源与食品工程学院云南高原生物资源保护与利用研究中心,云南曲靖655011

出　　处：《北方园艺》2021年第2期53-59,共7页Northern Horticulture

基　　金：曲靖师范学院教育教学改革资助项目(JGXM2016013,JGXM201812);校企(曲靖博浩生物科技股份有限公司)合作资助项目;云南省教育厅资助项目(2020J0632)。

摘　　要：以色素万寿菊为试验材料,采用母本资源不育株叶片、带腋芽茎段和侧芽为外植体,对不同的培养基配方条件下的生长情况进行统计,研究了灭菌处理、生长调节剂组合、AgNO3对丛生芽诱导的影响,以期快速获得遗传稳定的万寿菊雄性不育植株,为杂交育种提供基础研究材料。结果表明:以带腋芽的茎段为最佳外植体,1 g·L^-1 HgCl2灭菌10~12 min的灭菌效果最好,B1-3较易诱导丛生芽,其最佳的诱导培养基为MS+1.0 mg·L^-16-BA+0.5 mg·L^-1 NAA+30 g·L^-1蔗糖+7 g·L^-1琼脂,诱导率高达93%,2.0 mg·L^-1 AgNO3有利于丛生芽增殖,使B1-3丛生芽增殖率达151.6%,丛生芽生根培养后移栽成活率达95%以上。发现万寿菊离体培养过程中,外植体不同,其丛生芽诱导率不同,最佳外植体为带腋芽茎段。不同的灭菌剂的灭菌效果不同,1 g·L^-1 HgCl2灭菌效果最好;丛生芽诱导时6-BA与NAA不同浓度组合优于6-BA与IAA组合;低浓度AgNO3能够促进丛生芽诱导,高浓度反而会降低。Pigment marigold was selected as research object.Leaves,stem with axillary buds and lateral buds of sterile plants from pigment marigold mother resources were used to research the effects of sterilization treatment,growth regulator combination and AgNO3 concentration on multiple shoots induction.The aim was to obtain the genetically stable male sterile plants of marigold,and provide basic research materials for cross breeding.The results showed that stem with axillary buds were the best explants.The best sterilization agent was 1 g·L^-1 HgCl2 to sterilize 10^-12 minutes.B1-3 was easy to induce multiple shoots,MS+1.0 mg·L^-16-BA+0.5 mg·L^-1 NAA+30 g·L^-1 sucrose+7 g·L^-1 agar was optimal induction medium,the induction rate for B1-3 was up to 93%.2.0 mg·L^-1 AgNO3 was beneficial to proliferation of multiple shoots,and the proliferation rate of B1-3 was to 151.6%.After rooting culture,the survival rate of transplanting was over 95%.Invitroculture of marigold,the rate of cluster bud induction was different with different explants.Stem with axillary buds were the best explants.Different sterilization agents had different influenceson sterilization effect,1 g·L^-1 HgCl2 of sterilization effect was the best.The combination of 6-BA and NAA was better than that of 6-BA and IAA for cluster buds induction.Low concentration AgNO3 could promote the induction of cluster buds,but high concentrations had opposite effect.

关 键 词：色素万寿菊 离体快繁 雄性不育系 

分 类 号：S681.9[农业科学—观赏园艺]