毛竹茎秆快速生长期光合关键酶活性及基因表达分析  

Activities of key enzymes involved in photosynthesis and expression patterns of corresponding genes during rapid growth of Phyllostachys edulis

作　　者：王灵杰 栗青丽 高培军[1,2] 韦赛君 吕嘉欣 高岩[1,2] 张汝民[1,2] WANG Lingjie;LI Qingli;GAO Peijun;WEI Saijun;LÜJiaxin;GAO Yan;ZHANG Rumin(School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江杭州311300 [2]浙江农林大学亚热带森林培育国家重点实验室,浙江杭州311300

出　　处：《浙江农林大学学报》2021年第1期84-92,共9页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31570686);浙江省省院合作项目(2018SY07)。

摘　　要：【目的】揭示毛竹Phyllostachys edulis快速生长期茎秆光合碳同化规律。【方法】以毛竹笋竹为试材,采用分光光度法测定了毛竹茎秆和成熟叶片核酮糖-1,5-二磷酸氧合酶(Rubisco)、磷酸烯醇式丙酮酸羧化酶(PEPC)、NADP-苹果酸酶(NADP-ME)、NADP-苹果酸脱氢酶(NADP-MDH)、PEP羧激酶(PEP-CK)、磷酸烯醇式丙酮酸双激酶(PPDK)活性,运用实时荧光定量聚合链式反应(qPCR)对相应部位光合关键酶基因相对表达量进行分析。【结果】茎秆7~19节间Rubisco活性均极显著低于叶片(P<0.01),随着节间的升高,Rubisco活性逐渐降低;茎秆中PEPC、NADP-ME、NADP-MDH、PPDK活性在第7节间最高,分别是叶片的3.01倍、5.69倍、4.46倍、4.05倍(P<0.01),随着节间的升高,酶活性均极显著降低(P<0.01)。茎秆中PePEPC、PeNADP-ME、PeNADP-MDH、PePPDK基因表达量在第7节间最高,分别是叶片的3.48、7.89、6.48、3.46倍(P<0.01),随着节间的升高,这些基因表达量均极显著降低(P<0.01)。【结论】毛竹快速生长期茎秆主要以NADP-ME和NAD-ME途径对竹腔内部高浓度二氧化碳(CO2)再固定,减少自身碳损耗,形成的碳水化合物被茎秆快速生长再利用。[Objective]The aim is to reveal the law of photosynthetic carbon assimilation of Phyllostachys edulis stem during rapid growth period.[Method]The activities of ribulose-1,5-diphosphate oxidase(Rubisco),phosphoenolpyruvate carboxylase(PEPC),NADP-malate(NADP-ME),NADP-malate dehydrogenase(NADPMDH),PEP carboxykinase(PEP-CK)and phosphoene alcohol pyruvate double kinase(PPDK)in the stems of different internodes of Ph.edulis shoots and mature leaves were determined by spectrophotometry,the relative expression of photosynthetic key enzyme genes in the corresponding parts was analyzed by real-time fluorescence quantitative polymerase chain reaction(qPCR).[Result]The Rubisco activity of the 7−19 internodes of the stems was significantly lower than that of the leaves(P<0.01).With the increase of the internodes,the Rubisco activity gradually decreased first.The activities of PEPC,NADP-ME,NADP-MDH and PPDK in the stems were the highest among the 7th internodes,which were 3.01 times,5.69 times,4.46 times and 4.05 times of the leaves(P<0.01),with the increase of internodes,the activity of PEPC,PPDK,NADP-ME and NADP-MDH first significantly reduced(P<0.01).The gene expression levels of PePEPC,PeNADP-ME,PeNADP-MDH and PePPDK in the stems were the highest among the 7th nodes,which were 3.48 times,7.89 times,6.48 times and 3.46 times of the leaves(P<0.01).Increased,PePEPC,PeNADP-ME,PeNADP-MDH,PePPDK gene expression was significantly reduced(P<0.01).[Conclusion]The fast growing stems of Ph.edulis mainly fix the high concentration of CO2 in the bamboo cavity through the NADP-ME and NAD-ME pathways to reduce their own carbon loss,and the carbohydrates formed are rapidly grown and reused by the stalks.

关 键 词：毛竹 茎秆 光合酶 基因表达 C4途径 

分 类 号：S718.4[农业科学—林学]