水稻两系窄叶突变体Fz1S的表型分析与基因定位  

Phenotypic Analysis and Gene Mapping of Narrow Leaf Mutant FZ1S in Photoperiod-thermo Sensitive Genic Male Sterile Rice

作　　者：冯慧[1] 吴孝波[1] 黄强[1] 肖梅 刘育生[1] 杨成明[1] FENG Hui;WU Xiao-bo;HUANG Qiang;XIAO Mei;LIU Yu-sheng;YANG Cheng-ming(Sichuan Institute of Atomic Energy,Sichuan Chengdu 610101,China)

机构地区：[1]四川省原子能研究院,四川成都610101

出　　处：《西南农业学报》2020年第12期2702-2706,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省科技计划应用基础项目(2017JY0244);四川省辐射诱变技术育种平台(201NZ0106);主要农作物诱变育种项目“水稻核辐射诱变育种技术创新与品种创制”(2016YFD0102103);四川省科技计划项目“科技支撑计划引领性水稻育种材料与方法创新”(2016NYZ0028);四川省省级科研院所基本科研业务费项目“2019年,优质窄叶突変体Fz1S核心株系的提取及配合力研究”。

摘　　要：【目的】通过对水稻窄叶性状的研究,使水稻株型更加接近理想株型获得更高产量。【方法】本研究通过电子束辐照诱变深08S,获得了水稻窄叶突变体,经多代自交,其窄叶性状能够稳定遗传,命名为辐窄1S(Fz1S),对其进行了表型鉴定、细胞学观察、遗传分析和基因定位。【结果】与野生型深08S相比,该突变体剑叶、倒2叶叶片变窄、变短,植株变矮,穗子变短,千粒重降低,粒长、粒宽无显著变化。细胞学观察表明:叶片变窄主要是由于大、小维管束数量变少引起的。遗传分析表明:突变体辐窄1S(Fz1S)的突变性状受1对隐性基因控制。利用Mutmap测序定位突变基因,结果表明突变基因可能位于8号染色体,大致在4.9~5.3 Mb的区域。【结论】本研究结果为窄叶基因的克隆和功能分析奠定了良好基础,也为水稻株型改良提供了基因资源和育种材料。【Objective】 Through the study of narrow leaf trait, the rice plant shape was closer to the ideal plant shape to obtain higher yield.【Methods】In this study, the narrow leaf mutant was derived from the Photoperiod-thermo Sensitive Genic Male Sterile Shen08 S by electron beam irradiation, after multi-generation self-crossing, the narrow leaf trait could be inherited stably, named FZ1 S, the phenotype identification, cytological observation, genetic analysis and gene mapping were carried out. 【Results】Compared with the wild-type Shen08 S, the mutant flag leaves and second leaves became narrower and shorter, plant height became shorter, the panicle length became shorter, thousand seed weight decrease, and the grain length, grain width had no significant change. Cytological observations showed that the narrowing of leaves was mainly caused by the decrease in the number of large and small vascular bundles. Genetic analysis showed that the mutant trait was controlled by a single recessive genes. Mutmap sequencing was used to locate the mutated genes, indicating that the mutated genes was located in chromosome 8, approximately region was 4.9-5.3 Mb. 【Conclusion】The results of this study laid a good foundation for cloning and functional analysis of narrow-leaf genes and also provided the genetic resources and breeding materials for rice plant-shape improvement.

关 键 词：水稻 窄叶突变体 MutMap 离子束辐照诱变 

分 类 号：S511[农业科学—作物学]