温度响应上位抑制草莓开花基因FaDSF遗传模型分析  

Genetic Model Analysis of Gene FaDSF with Effect of Temperature Sensitively and Epistatically Supressing Flowering in Cutivated Strawberry

作　　者：阮继伟[1,2] 孙建丽 万红 单芹丽[1,2] 田林孟[2] 蒋海玉 杨春梅 RUAN Ji-wei;SUN Jian-li;WAN Hong;SHAN Qin-Li;TIAN Lin-meng;JIANG Hai-yu;YANG Chun-Mei(Flower Research Institute.,Yunnan Academy of Agricultural Sciences/National Engineering and Technology Research Center for Orna­mental Horticulture,Yunnan Kunming 650205,China;Yuxiyunxing Biotechnology Co.,Ltd.,Yunnan Yuxi 652600,China;Horti­culture Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China;Agriculture Economics and In­formation Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China)

机构地区：[1]云南省农业科学院花卉研究所/国家观赏园艺工程技术研究中心,云南昆明650205 [2]玉溪云星生物科技有限公司,云南玉溪652604 [3]云南省农业科学院园艺研究所,云南昆明650205 [4]云南省农业科学院农业经济与信息研究所,云南昆明650205

出　　处：《西南农业学报》2020年第12期2921-2926,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(31860534);云南省农业科学院重点基金(YJZ201702)。

摘　　要：【目的】构建抑制开花基因FaDSF的分离群体,分析并验证其遗传模型,为下一步开展FaDSF基因定位和分子机理研究奠定基础。【方法】利用不同年份2次杂交构建的‘章姬’בAlbion’群体,母本是季节性草莓,父本是四季草莓。在播种当年及越冬后的2个年份,调查群体内表现四季性和季节性植株的分离比例,分析温度响应上位抑制开花基因FaDSF的分离规律和遗传模型。【结果】不同年份杂交构建的‘章姬’בAlbion’遗传群体,播种当年四季性和季节性植株的分离比例均符合1∶3的分离模型,而在越冬后的年份四季性和季节性植株的分离比例均符合1∶1的分离模型。【结论】在‘章姬’בAlbion’杂交群体中存在温度响应抑制开花基因FaDSF分离群体,其显性并上位抑制FaPFRU基因。这为开展FaDSF基因定位和分子生物学研究提供了遗传群体和遗传模型;也为研究温度影响草莓开花的分子机理提供了一个切入点。【Objective】To establish segregated F1 population, and to analyze and validate the inheritance model of FaDSF, a gene suppressing flowering, so as to facilitate the future researches on gene mapping and molecular mechanism.【Method】Two F1 population of‘Akihime’ × ‘Albion’ were established in different years.‘Akihime’ was seasonal-flowering(SF) and ‘Albion’was perpetual-flowering(PF). The flowering phenotype of each plant from F1 population of ‘Akihime’ × ‘Albion’ were investigated in sowing years and the following 2 years. The segregated ratios of PF and SF plants were analyzed, and inheritance model of FaDSF was predicted and validated. 【Result】Two F1 populations of ‘Akihime’ × ‘Albion’ were established in different years, and the segregated ratios of SF∶PF fitted the model of 1∶3 in the sowing years, while the segregated ratios of SF∶PF fitted the model of 1∶1 in the following years.【Conclusion】There were segregated populations of FaDSF in F1 population of‘Akihime’ × ‘Albion’, and FaDSF was temperature-sensitively dominantly suppressing the FaPFRU. This research would facilitate the future researches on gene mapping and molecular mechanism of FaDSF, which also provided a cut-in point to research the mechanism on how temperature affect flowering of strawberry.

关 键 词：凤梨草莓 表观遗传 四季性 遗传群体 表型 

分 类 号：S668[农业科学—果树学]