3种提取方法对二黄汤中5种成分在大鼠体内药动学的影响  

Effects of three extraction methods on the pharmacokinetics of five constituents in Erhuang Decoction in rats in vivo

作　　者：王京龙 于定荣[2] 张超[3] 王英姿[4] 孙秀梅[3] 张兆旺[3] WANG Jing-long;YU Ding-rong;ZHANG Chao;WANG Ying-zi;SUN Xiu-mei;ZHANG Zhao-wang(College of Food Sciences&Pharmaceutical Engineering,Zaozhuang University,Zaozhuang 277160,China;Institute of Chinese Material Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;College of Pharmacy,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]枣庄学院食品科学与制药工程学院,山东枣庄277160 [2]中国中医科学院中药研究所,北京100700 [3]山东中医药大学药学院,山东济南250355 [4]北京中医药大学药学院,北京100029

出　　处：《中成药》2021年第1期1-5,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81603298);山东省自然科学基金项目(ZR2018LH020);山东省高等学校科研计划项目(J18K035);山东省高等学校“青创科技计划”团队项目(2019KJM006)。

摘　　要：目的考察3种提取方法对二黄汤中甘草苷、黄芩苷、巴马汀、小檗碱、甘草次酸在大鼠体内药动学的影响。方法分别采用半仿生-酶法、半仿生法、醇提法提取二黄汤。大鼠灌胃给予提取液(≤1000 Da,15.0 g/kg)后,于0.083、0.167、0.333、0.5、1、2、3、4、6、8、12、24、48 h采血,HPLC-MS/MS法测定5种成分血药浓度,计算主要药动学参数。结果半仿生提取液中甘草苷tmax更短,Cmax、AUC0~t更高;半仿生-酶提取液中黄芩苷、巴马汀、小檗碱、甘草次酸Cmax、AUC0~t更高,其中巴马汀、小檗碱tmax更短。结论半仿生-酶法、半仿生法有利于二黄汤中5种成分的提取,可提高其口服吸收体内生物利用度。AIM To investigate the effects of three extraction methods on the pharmacokinetics of liquiritin,baicalin,palmatine,berberine and glycyrrhizic acid in Erhuang Decoction in rats in vivo.METHODS(Semi-bionic)-enzyme method,semi-bionic method and ethanol extraction method were adopted in the extraction for Erhuang Decoction,respectively.Rats were given intragastrical administration of extractions(≤1000 Da,15.0 g/kg),after which blood collection was made at 0.083,0.167,0.333,0.5,1,2,3,4,6,8,12,24,48 h,HPLC-MS/MS was adopted in the plasma concentration determination of five constituents,and main pharmacokinetic parameters were calculated.RESULTS In the semi-bionic extract,liquiritin demonstrated shorter tmax and higher Cmax,AUC0-t.In the(semi-bionic)-enzyme extract,baicalin,palmatine,berberine and glycyrrhizic acid displayed higher Cmax and AUC0-t,among which palmatine and berberine exhibited shorter tmax.CONCLUSION(Semi-bionic)-enzyme method and semi-bionic method are beneficial to the extraction for five constituents in Erhuang Decoction and can enhance their in vivo bioavailabilities of oral administration.

关 键 词：二黄汤 半仿生-酶法 半仿生法 醇提法 化学成分 药动学 HPLC-MS/MS 

分 类 号：R969.1[医药卫生—药理学]