UHPLC-PDA法同时测定毛大丁草中6种成分  

Simultaneous determination of six constituents in Gerbera piloselloides by UHPLC-PDA

作　　者：游景瑞 孙佳[3] 兰燕宇[1] 李勇军[1] 王永林[3] 刘春花[1] YOU Jing-rui;SUN Jia;LAN Yan-yu;LI Yong-jun;WANG Yong-lin;LIU Chun-hua(State Key Laboratory of Functions and Applications of Medicinal Plants,Engineering Research Center for Development and Application of Ethnic Medicine and TCM(Ministry of Education),Guizhou Medical University,Guiyang 550004,China;School of Pharmacy,Guizhou Medical University,Guiyang 550025,China;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学民族药与中药开发应用教育部工程研究中心,省部共建药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550025 [3]贵州医科大学,贵州省药物制剂重点实验室,贵州贵阳550004

出　　处：《中成药》2021年第1期111-116,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82060777,U1812403);中央引导地方科技专项项目(黔科中引地[2018]4006);贵州省自然科学基金资助项目(黔科合基础[2018]1128);贵州省科学技术厅项目(黔科合平台人才[2016]5613、5677,黔科合平台人才[2017]5718);贵州省国家级、省级创新创业训练计划项目(DC 201710660006);贵州医科大学博士启动基金(YJ2017-12)。

摘　　要：目的建立UHPLC-PDA法同时测定毛大丁草Gerbera piloselloides(Linn.)Cass.中熊果苷、绿原酸、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C的含量。方法毛大丁草50%甲醇提取物的分析采用Waters BEH C18色谱柱(2.1 mm×150 mm,1.7μm);流动相乙腈-0.1%甲酸,梯度洗脱;体积流量0.28 mL/min;柱温40℃;检测波长280、328 nm。结果熊果苷、绿原酸、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C分别在4.45~284.59(r=0.9999)、0.30~19.52(r=0.9998)、0.30~19.50(r=0.9998)、0.31~19.84(r=0.9999)、0.61~38.96(r=0.9998)、0.32~20.19(r=0.9998)μg/mL范围内线性关系良好,平均加样回收率分别为102.06%、103.37%、104.97%、104.79%、105.21%、104.75%,RSD分别为1.88%、1.70%、1.20%、1.89%、1.53%、1.25%。结论该方法稳定,重复性好,可用于毛大丁草的质量控制。AIM To establish a UHPLC analysis method for simultaneous determination of arbutin,chlorogenic acid,luteoloside,isochlorogenic acid B,isochlorogenic acid A and isochlorognic acid C in Gerbera piloselloides(Linn.)Cass..METHODS The analysis of 50%methanol extract of G.piloselloides was performed on a 40℃thermostatic Waters BEH C18 column(2.1 mm×150 mm,1.7μm),with the mobile phase comprising of acetonitrile and 0.1%formic acid flowing at 0.28 mL/min in a gradient elution manner,and the detection wavelengths were set at 280,328 nm.RESULTS Arbutin,chlorogenic acid,luteoloside,isochlorogenic acid B,isochlorogenic acid A and isochlorognic acid C showed good linear relationships within the ranges of 4.45-284.59(r=0.9999),0.30-19.52(r=0.9998),0.30-19.50(r=0.9998),0.31-19.84(r=0.9999),0.61-38.96(r=0.9998),0.32-20.19(r=0.9998)μg/mL,whose average recoveries were 102.06%,103.37%,104.97%,104.79%,105.21%,104.75%with the RSDs of 1.88%,1.70%,1.20%,1.89%,1.53%,1.25%,respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G.piloselloides.

关 键 词：毛大丁草 化学成分 UHPLC 

分 类 号：R284.1[医药卫生—中药学]