microRNA-146a对牙龈卟啉单胞菌脂多糖刺激下淋巴细胞分泌细胞因子的调节作用  

MicroRNA-146a regulates the production of cytokines in lymphocytes stimulated by Porphyromonas gingivalis lipopolysaccharide

作　　者：司雨婷 宋金花 方珍[2] 韩晓哲[3] 蒋少云[1,2] Si Yuting;Song Jinhua;Fang Zhen;Han Xiaozhe;Jiang Shaoyun(Dept.of Periodontology,Hospital of Stomatology,Tianjin Medical University,Tianjin 300070,China;Dept.of Periodontology,Stomatological Center,Peking University Shenzhen Hospital,Shenzhen 518036,China;The Forsyth Institute,Harvard School of Dental Medicine,Cambridge 02146,USA)

机构地区：[1]天津医科大学口腔医院牙周科,天津300070 [2]北京大学深圳医院口腔医学中心牙周科,深圳518036 [3]美国哈佛大学牙学院Forsyth研究所,美国剑桥02146

出　　处：《华西口腔医学杂志》2021年第1期26-31,共6页West China Journal of Stomatology

基　　金：国家自然科学基金(81670990);北京大学深圳医院院内基础研究基金(JCYJ2019004RC);美国国立口腔与颅面研究基金(R01DE025255)。

摘　　要：目的探讨microRNA-146a(miR-146a)对牙龈卟啉单胞菌(P.gingivalis)脂多糖(LPS)刺激下淋巴细胞分泌细胞因子的作用。方法从小鼠脾脏收集淋巴细胞。实时定量聚合酶链反应和酶联免疫反应用于检测淋巴细胞在P.gingivalis LPS、miR-146a模拟物或抑制剂处理后细胞因子的表达。结果与P.gingivalis LPS未刺激组相比,P.gingivalis LPS能促进白细胞介素(interleukin,IL)-1β、IL-6、细胞核因子κB受体活化因子配体(RANKL)和IL-10表达(P<0.05),虽抑制骨保护素(OPG)mRNA水平(P<0.05),但分泌水平差异无统计学意义(P>0.05)。与阴性对照组相比,P.gingivalis LPS处理的淋巴细胞中miR-146a模拟物抑制IL-1β、IL-6和RANKL表达(P<0.05),促进IL-10和OPG表达(P<0.05),miR-146a抑制剂对这些细胞因子产生相反的效果(P<0.05)。结论miR-146a可通过抑制淋巴细胞IL-1β、IL-6和RANKL表达,促进IL-10和OPG表达,从而抑制P.gingivalis LPS的炎性作用,为骨改建提供良好的成骨环境。Objective This study aimed to investigate the effects of microRNA-146a(miR-146a)on the production of cytokines in lymphocytes stimulated by Porphyromonas gingivalis(P.gingivalis)lipopolysaccharide(LPS).Methods Lymphocytes were harvested from mouse spleen and cultured in vitro.The cells were treated with P.gingivalis LPS,miR-146a mimic,or miR-146a inhibitor.Scramble RNA served as the negative control of mimic and inhibitor.The production of inflammatory cytokines was detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay.Results Compared with non-LPS-stimulated group,P.gingivalis LPS could increase the levels of interleukin(IL)-1β,IL-6,receptor activator NF-κB ligand(RANKL),and IL-10(P<0.05)and decrease the mRNA level of osteoprotectin(OPG)(P<0.05).However,it did not significantly change the secretion of OPG.Compared with the negative control group,miR-146a mimic upregulated the levels of IL-10 and OPG(P<0.05),downregulated IL-1β,IL-6,and RANKL(P<0.05).Meanwhile,miR-146a inhibitor had a reverse effect on these cytokines(P<0.05)in P.gingivalis LPS-treated-lymphocytes.Conclu⁃sion MiR-146a can provide a suitable microenvironment for bone formation by preventing the inflammatory effects of P.gingivalis LPS through the inhibition of IL-1β,IL-6,and RNAKL,thereby enhancing IL-10 and OPG.

关 键 词：牙周炎 microRNA-146a 细胞因子 淋巴细胞 脂多糖 

分 类 号：Q257[生物学—细胞生物学]